Abstract

Xylose glucosides (XG_s) have been effectively used in many industries because of their resistance to browning reactions and acids and prebiotic properties. In this study, XG_s were synthesized through the transglycosylation reaction of recombinant amylomaltase. The optimal conditions for synthesis included the incubation in 1.5% (wt/vol) tapioca starch, 2% (wt/vol) xylose and 4 U/mL of amylomaltase in 20 mM Tris-HCl buffer, pH 7.0 at 70°C for 120 h. Two products had Mw of 335.10 and 497.20 Da according to mass spectrometry analysis. These mass values corresponded to xylose monoglucoside (XG₁) and xylose maltoside (XG₂). The determination of XG₁ structure was performed by ¹H and ¹³C-NMR analysis to identify α-1,4-glycosidic bond. The long-carbohydrate chains of xylose were less effective in sweetness property and acidic resistance than xylose and sucrose. However, XG₁ and XG₂ showed more solubility, resistance to browning reactions and prebiotic properties than xylose. Thus, the XG_s have the potential as functional ingredients for health benefits.

中文翻译：

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酶法合成功能性木糖葡糖苷及其在益生元中的应用

摘要

木糖苷（XG _s）由于其对褐变反应，酸和益生元特性的抵抗力，已在许多行业中得到有效使用。在这项研究中，XG_小号是通过重组淀粉麦芽糖酶的糖转移反应合成。合成的最佳条件包括在20 mM Tris-HCl缓冲液（pH 7.0）中于70°C在120°M的条件下于1.5％（wt / vol）的木薯淀粉，2％（wt / vol）的木糖和4 U / mL的淀粉淀粉酶中孵育120分钟。 H。根据质谱分析，两种产物的Mw分别为335.10和497.20Da。这些质量值对应于木糖单葡糖苷（XG ₁）和木糖麦芽糖苷（XG ₂）。XG ₁结构的测定在¹ H下进行¹³ C-NMR分析鉴定α-1，4-糖苷键。木糖的长碳水化合物链的甜味性和耐酸性不如木糖和蔗糖。然而，与木糖相比，XG ₁和XG ₂显示出更高的溶解度，抗褐变反应性和益生元特性。因此，XG_小号有作为健康有益的功能性成分的潜力。