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Enzymatic Synthesis of Functional Xylose Glucoside and Its Application to Prebiotic
Applied Biochemistry and Microbiology ( IF 1.0 ) Pub Date : 2021-03-12 , DOI: 10.1134/s0003683821020058
N. Khummanee , P. Rudeekulthamrong , J. Kaulpiboon

Abstract

Xylose glucosides (XGs) have been effectively used in many industries because of their resistance to browning reactions and acids and prebiotic properties. In this study, XGs were synthesized through the transglycosylation reaction of recombinant amylomaltase. The optimal conditions for synthesis included the incubation in 1.5% (wt/vol) tapioca starch, 2% (wt/vol) xylose and 4 U/mL of amylomaltase in 20 mM Tris-HCl buffer, pH 7.0 at 70°C for 120 h. Two products had Mw of 335.10 and 497.20 Da according to mass spectrometry analysis. These mass values corresponded to xylose monoglucoside (XG1) and xylose maltoside (XG2). The determination of XG1 structure was performed by 1H and 13C-NMR analysis to identify α-1,4-glycosidic bond. The long-carbohydrate chains of xylose were less effective in sweetness property and acidic resistance than xylose and sucrose. However, XG1 and XG2 showed more solubility, resistance to browning reactions and prebiotic properties than xylose. Thus, the XGs have the potential as functional ingredients for health benefits.



中文翻译:

酶法合成功能性木糖葡糖苷及其在益生元中的应用

摘要

木糖苷(XG s)由于其对褐变反应,酸和益生元特性的抵抗力,已在许多行业中得到有效使用。在这项研究中,XG小号是通过重组淀粉麦芽糖酶的糖转移反应合成。合成的最佳条件包括在20 mM Tris-HCl缓冲液(pH 7.0)中于70°C在120°M的条件下于1.5%(wt / vol)的木薯淀粉,2%(wt / vol)的木糖和4 U / mL的淀粉淀粉酶中孵育120分钟。 H。根据质谱分析,两种产物的Mw分别为335.10和497.20Da。这些质量值对应于木糖单葡糖苷(XG 1)和木糖麦芽糖苷(XG 2)。XG 1结构的测定在1 H下进行13 C-NMR分析鉴定α-1,4-糖苷键。木糖的长碳水化合物链的甜味性和耐酸性不如木糖和蔗糖。然而,与木糖相比,XG 1和XG 2显示出更高的溶解度,抗褐变反应性和益生元特性。因此,XG小号有作为健康有益的功能性成分的潜力。

更新日期:2021-03-12
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