Abstract

In order to identify qualitative markers of a potential mycoherbicide against Canada thistle based on the mycelium of the fungus Stagonospora cirsii VIZR 1.41, changes in its pathogenicity and lipid profiles during submerged cultivation on liquid sucrose-soybean medium were analyzed. The fatty-acid composition of major lipids and the identification of molecular species of structural lipids were determined. The lipid composition during fungal growth changed together with its pathogenicity. The highly pathogenic mycelium of the fungus (at the log phase of growth) was characterized with the relatively highest content of structural lipids: phosphatidylcholine (PC), phosphatidylethanolamine (PE), and ergosterol (ES). Their relative concentration decreased with culture age and a decrease in pathogenicity. The highest lipid yield was found at the beginning of stationary growth phase (at the maximal biomass yield) of S. cirsii; triacylglycerides (TAGs) were the most abundant. The double-bond index of fatty acids in glycerolipids decreased with culture age, and the substitution of molecular PC species (34:2 versus 36:4) and PE (36:4 versus 34:2) was observed as well. High levels of ES, TAG, PC (in particular, 34:2 forms) and PE (36:4 forms) were presumed to be qualitative markers of S. cirsii mycelium.

中文翻译：

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淹没栽培中真菌Stagonospora cirsii VIZR 1.41的菌丝体致病性和脂质组成

摘要

为了基于真菌Stagonospora cirsii的菌丝体鉴定针对加拿大蓟的潜在除草剂的定性标记分析了VIZR 1.41，在液体蔗糖-大豆培养基上深水培养过程中的致病性和脂质谱变化。确定了主要脂质的脂肪酸组成和结构脂质的分子种类的鉴定。真菌生长过程中的脂质组成及其致病性发生了变化。真菌的高致病性菌丝体（在生长的对数期）具有相对最高的结构脂质含量：磷脂酰胆碱（PC），磷脂酰乙醇胺（PE）和麦角固醇（ES）。它们的相对浓度随着培养年龄的增长和致病性的降低而降低。在西氏链球菌的固定生长期开始时（以最大的生物量产量）发现了最高的脂质产量。; 甘油三酯（TAGs）含量最高。甘油脂中脂肪酸的双键指数随培养年龄而降低，并且还观察到了分子PC种类（34：2对36：4）和PE（36：4对34：2）的取代。高水平的ES，TAG，PC（尤其是34：2形式）和PE（36：4形式）被认为是柠檬链球菌菌丝的定性标记。