With increasing demand for biobased materials, major importance was given to the extraction and application of polysaccharides issued from renewable biomasses like algae. In this work, we investigate the feasibility of elaborating an active ulvan-based biomembrane prepared from stranded Ulva sp. biomass. Variations of extraction parameters showed that the optimal yield (13.8% w/w) was obtained using methanol under Soxhlet extraction for the defatting step and 5% (w/v) of ammonium oxalate as chelating reagent. The molecular weight (MW) was determined by gel permeation chromatography (GPC), which showed variability of MW from 201 to 1841 kDa depending on the extraction procedure. The monosaccharide composition of the obtained high MW ulvan showed the presence of rhamnose (17.61 ± 0.5%), xylose (9.22 ± 0.56%), and glucuronic acid (24.86 ± 1.29%). Purity and quality of ulvan were also assessed by FTIR and TGA analyses. The high MW ulvan fraction was thereafter used for the synthesis of a polyelectrolyte-type ulvan/chitosan biomembrane at low KCl concentration. The obtained membrane was then characterized using FTIR and XPS analyses, which evidenced that the ulvan sulfur groups were implicated in the interaction with chitosan chains. Finally, to assess the biological properties of the biomembrane, anticoagulant behavior was evaluated using APTT assay, and it has been found to significantly increase the blood clotting time in comparison with controls. This work shows the proof of concept of ulvan conversion to high added value products and, therefore, consolidates the implementation of cost-effective biorefinery approaches of green macroalgal biomass.

随着对生物基材料的需求的增加，从藻类等可再生生物质中产生的多糖的提取和应用变得尤为重要。在这项工作中，我们调查了从搁浅的Ulva制备活性的基于ulvan的生物膜的可行性sp。生物质。提取参数的变化表明，在脱脂步骤中使用甲醇在索格利特萃取条件下，以5％（w / v）的草酸铵作为螯合剂，可获得最佳收率（13.8％w / w）。分子量（MW）通过凝胶渗透色谱法（GPC）测定，其显示出从201至1841kDa的MW变异性，这取决于提取程序。所得高分子量ulvan的单糖组成显示鼠李糖（17.61±0.5％），木糖（9.22±0.56％）和葡萄糖醛酸（24.86±1.29％）的存在。还通过FTIR和TGA分析评估了ulvan的纯度和质量。此后，将高分子量ulvan馏分用于在低KCl浓度下合成聚电解质型ulvan /壳聚糖生物膜。然后使用FTIR和XPS分析对获得的膜进行表征，这表明ulvan硫基团与壳聚糖链的相互作用有关。最后，为了评估生物膜的生物学特性，使用APTT分析法评估了抗凝剂的行为，发现与对照相比，它显着增加了凝血时间。这项工作证明了将ulvan转化为高附加值产品的概念的证明，因此，巩固了绿色大型藻类生物质经济高效的生物精炼方法的实施。