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In vitro morphophysiological screening of drought-tolerant potato genotypes
In Vitro Cellular & Developmental Biology - Plant ( IF 2.2 ) Pub Date : 2021-03-11 , DOI: 10.1007/s11627-020-10155-z
M. Harun-Or-Rashid , M.A. Bari Miah , S.M. Shahinul Islam

Sixteen potato genotypes were assessed for drought tolerance through morphophysiological characteristics using stress tolerance trait index (STTI), principal component analysis (PCA), and cluster analysis (CA). Two to three wk old nodal segments explants (1.0 to 2.0 cm long) derived from in vitro grown sprouts were cultured on MS medium that contained with different concentrations of sorbitol. After 6 wk of culture initiation, the length of shoots, roots, and internodes; thickness of shoot and roots; number of leaves and roots; fresh and dry weight of whole plant; and water content of plants were recorded. For PCA, the cultivars Arun, Granola, Surjomukhi, Sheelbilati, Jamalu, Chollisha, Patnai, and Dohazari showed relatively higher values regarding drought tolerance and Diamant, Asterix, Courage, Cardinal, Lalpakri, Sindurkouta, Ausha, and Sadaguti were drought-sensitive cultivars. For cluster analysis, the cultivars Arun, Granola, and Surjomukhi showed high tolerances to drought and Diamant was sensitive. Analysis of variance (ANOVA), Duncan’s multiple range test (DMRT), and correlation coefficient for different morphological and physiological traits showed significantly higher values at p < 0.01 level among the tested genotypes. The protocols standardized under this study may be helpful for plant breeders and biotechnologist to develop mass production of potato seeds/tubers in drought-prone areas.



中文翻译:

耐旱马铃薯基因型的体外形态生理筛选

使用胁迫耐受性状指数(STTI),主成分分析(PCA)和聚类分析(CA)通过形态生理特征评估了十六种马铃薯基因型的耐旱性。体外衍生的两到三个wk的旧节段外植体(长1.0至2.0 cm)在含有不同浓度山梨糖醇的MS培养基上培养生长的芽苗。培养开始6周后,芽,根和节间的长度;芽和根的厚度;叶和根的数量;整株的鲜重和干重;并记录植物的水分。对于PCA,Arun,Granola,Surjomukhi,Sheelbilati,Jamalu,Chollisha,Patnai和Dohazari品种表现出相对较高的抗旱性,而Diamant,Asterix,Courage,Cardinal,Lalpakri,Sindurkouta,Ausha和Sadaguti是对干旱敏感的品种。为了进行聚类分析,品种Arun,Granola和Surjomukhi显示出对干旱的高耐受性,而Diamant非常敏感。方差分析(ANOVA),邓肯多范围检验(DMRT), 在测试的基因型中,p <0.01水平。在这项研究下标准化的协议可能对植物育种者和生物技术人员在干旱多发地区发展马铃薯种子/块茎的大规模生产有帮助。

更新日期:2021-03-12
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