The study highlights the fungal diversity of the traditional Algerian date’s product “Btana” prepared with direct Btana method (DBM) and undirect Btana method (UBM). Btana fungal populations were analyzed through 28S metabarcoding. Data treatment resulted in 122,997 reads representing three Phyla in which 76% reads (46 OTUs) belong to Ascomycota phylum. Zygosaccharomyces rouxii was the most prevailed species accounting for 35.40% of the total population. Similarity percentage analysis revealed a low level of resemblance in species in each of the two Btana types (DBM: 17.26%, UBM: 16.87). According to HPLC analysis, lactate was detected in nine samples within a range of 0.87-23.06 g/100g. Culture plating and subsequent D1/D2 domain of 28s DNA analysis showed the prevalence of Z. rouxii. Fermentation of non-renewed date medium revealed a high ethanol production (21.31 ± 2.89 g/100g) by Lachancea thermotolerans and 5.87 g/100g of lactates by Kluyveromyces delphensis. Enzymatic assay revealed a high esterase (C4) and naphtol-AS-BI-phosphohydrolase activity by L. thermotolerans, K. delphensis, and Pichia subpelliculosa, while a high level of α-fucosidase was recorded for L. thermotolerans and P. subpelliculosa. The current results demonstrated that the traditional date product Btana is a promising source for yeasts useful in production of value-added products like bioethanol and lactic acid using low-income date cultivars.

该研究强调了用直接 Btana 方法 (DBM) 和非直接 Btana 方法 (UBM) 制备的传统阿尔及利亚椰枣产品“Btana”的真菌多样性。通过 28S 元条形码分析 Btana 真菌种群。数据处理导致 122,997 个读数代表三个门，其中 76% 读数（46 个 OTU）属于子囊菌门。Zygosaccharomyces rouxii是最普遍的物种，占总种群的35.40%。相似性百分比分析显示，两种 Btana 类型中的每一种的物种相似度都较低（DBM：17.26%，UBM：16.87）。根据 HPLC 分析，在 0.87-23.06 g/100g 范围内的 9 个样品中检测到乳酸。培养板和随后的 28s DNA 分析的 D1/D2 域显示Z. rouxii的流行. 未经更新的枣培养基的发酵表明，耐热Lachancea 的 乙醇产量较高（21.31 ± 2.89 g/100g），而Kluyveromyces delphensis 的乳酸产量为 5.87 g/100g 。酶测定揭示了高酯酶（C4）和萘酚AS-BI-磷酸水解酶的活性通过L. thermotolerans，K. delphensis，和毕赤酵母属subpelliculosa，而α岩藻糖苷酶的高水平被记录为L. thermotolerans和P. subpelliculosa . 目前的结果表明，传统的枣制品 Btana 是酵母的有前途的来源，可用于使用低收入枣品种生产生物乙醇和乳酸等增值产品。