Copper nanoparticles (Cu-NPs) are water pollutants, which has significant toxic environmental effects. However, the effects of Cu-NPs on Takifugu fasciatus are still relatively unknown. Compared with the control (no add CuSO₄ and Cu-NPs). This study aims to describe the pathological changes in the gill and liver of pufferfish exposed to CuSO₄ (Cu²⁺ for short) and Cu-NPs dissolved at a concentration of either 20 or 100 μg/L, in a semi-static waterborne exposure regime for 28 days. All Cu treatments resulted in organ injuries, and the pathologies observed following exposure to Cu-NPs were broadly of the same type as those observed following exposure to Cu²⁺. In the gill, these included lamellar fusion, clubbed tips, and mucocytes swollen, and in the liver included oedema, aggregation of blood cells, and necrosis. Furthermore, enzyme activity assays revealed that Ca²⁺ -ATP activity significantly increased under the influence of Cu, whilst Na⁺-K⁺-ATPase activity was significantly inhibited. In this study, the CRT in T. fasciatus (referred to as TfCRT) was identified and full-length cDNA sequences were characterized. Subsequently, RT-PCR and western blot analysis were carried out to assess the expressions of TfCRT in the gill and liver at both the mRNA and protein levels. Under both the Cu-NPs and Cu²⁺ treatments, both the mRNA and protein expressions of TfCRT were considerably higher in the gill and liver compared to that of the control treatment. Furthermore, there were no significant differences between Cu-NPs and Cu²⁺ treatment (at the same treatment concentration) in the expressions of TfCRT at the mRNA or protein levels. Overall, both Cu²⁺ and Cu-NPs had negative effects on T. fasciatus, and Cu-NPs had the toxic effects similar to dissolved Cu. Our results provide the ecological safety data service of Cu-NPs in water, and provide a reference for the health T. fasciatus aquaculture.

铜纳米颗粒（Cu-NPs）是水污染物，具有明显的毒性环境影响。然而，Cu-NPs对T筋草的作用仍然相对未知。与对照相比（不添加CuSO ₄和Cu-NP）。这项研究旨在描述在半静态水性暴露下暴露于CuSO ₄（简写为Cu ²⁺）和溶解浓度为20或100μg/ L的Cu-NPs的河豚g和肝脏的病理变化政权为期28天。所有的铜治疗均导致器官损伤，暴露于铜纳米颗粒后观察到的病理类型与暴露于铜^2+后观察到的病理类型大致相同。在the中，这些包括层状融合，棍状尖端和粘液细胞肿胀；在肝脏中，包括水肿，血细胞聚集和坏死。此外，酶活性测定表明，在Cu的影响下，Ca ²⁺ -ATP活性显着增加，而Na ⁺ -K ⁺ -ATPase活性被显着抑制。在这项研究中，在CRT T.鲀（被称为TF CRT）被鉴定和全长cDNA序列进行了表征。随后，进行了RT-PCR和蛋白质印迹分析，以评估Tf CRT在g和肝脏中的mRNA和蛋白质水平的表达。在Cu-NPs和Cu ^2+下与对照组相比，在f和肝脏中，Tf CRT的mRNA和蛋白表达均显着较高。此外，在mRNA或蛋白质水平下，Tf CRT的表达在Cu-NPs和Cu ²⁺处理（在相同的处理浓度下）之间没有显着差异。总体而言，两者的Cu ²⁺和Cu-的NP对负面影响T.鲀，和Cu-纳米粒具有类似于溶解铜的毒性作用。我们的结果为水中铜-NPs的生态安全数据服务提供了参考，并为健康的筋膜水。养殖提供了参考。