ABSTRACT

The survival of Salmonella in subtropical river water depends on genetic and metabolic reorganization for the expression of alternative metabolic pathways in response to starvation, which allows Salmonella to use environmental carbon sources (C-sources). However, knowledge regarding the metabolic plasticity of Salmonella serotypes for C-source utilization when exposed to these conditions remains unclear. The aim of this study was to evaluate the metabolic response and level of environmental C-source consumption by environmental Salmonella (Oranienburg and Saintpaul) and clinical Salmonella (Typhi) serotypes by comparing laboratory growth against exposure to river water conditions. Metabolic characterization was performed using a Biolog® EcoPlate^TM containing 31 C-sources. The results obtained under laboratory growth conditions showed that environmental serotypes used 74.1% of the C-sources, whereas the clinical serotype used 45.1%. In contrast, in river water, all strains used up to 96.7% of the C-sources. Salmonella exposure to river water increases its capacity to use environmental C-sources.

摘要

沙门氏菌在亚热带河水中的生存依赖于基因和代谢重组，以表达替代代谢途径以应对饥饿，这使沙门氏菌能够利用环境碳源（C-sources）。然而，关于沙门氏菌血清型在暴露于这些条件下用于 C 源利用的代谢可塑性的知识仍不清楚。本研究的目的是评估环境沙门氏菌（奥拉宁堡和圣保罗）和临床沙门氏菌对环境碳源消耗的代谢反应和水平(Typhi) 血清型通过比较实验室生长与暴露于河水条件。使用含有 31个碳源的 Biolog® EcoPlate ^{TM进行代谢表征。}在实验室生长条件下获得的结果表明，环境血清型使用了 74.1% 的 C 源，而临床血清型使用了 45.1%。相比之下，在河水中，所有菌株使用了高达 96.7% 的 C 源。沙门氏菌暴露于河水中会增加其利用环境碳源的能力。