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Detection of polyomavirus microRNA-5p expression in saliva shortly after kidney transplantation
Journal of Oral Microbiology ( IF 3.7 ) Pub Date : 2021-03-12 , DOI: 10.1080/20002297.2021.1898838
Ana Carolina Mamana 1 , Maria Stincarelli 2 , Dmitry José De Santana Sarmento 3 , Alexandre Mendes Batista 1 , Tânia Regina Tozetto-Mendoza 1 , Marina Gallottini 3 , José Osmar Medina De Abreu Pestana 4 , Paulo Henrique Braz-Silva 1, 3 , Simone Giannecchini 2
Affiliation  

ABSTRACT

Background: MicroRNAs (miRNAs) of polyomavirus (PyV) are present in several biological fluids and are suggested to be relevant viral factors for monitoring its persistence.

Aim: To evaluate the effect of an immunosuppressive regimen on the status of PyV-miRNA-5p in the oral cavity.

Materials and Methods: The JCPyV, BKPyV, MCPyV miRNA-5p were investigated in paired saliva and plasma samples obtained from 23 patients before and shortly after renal-transplantation by using real-time RT-PCR.

Results: Overall, within a short-time after transplantation, patients exhibited decreased numbers of leukocyte and lymphocyte as well as low levels of creatinine. During the clinical management of the patients, a significant amount of saliva samples were positive for JCPyV and BKPyV miRNA-5p (range: 26%-91%) compared to paired plasma samples (range: 9%-35%). Among the two polyomaviruses showing positive expression of miRNA-5p, BKPyV presented the highest positivity in saliva (91%) and MCPyV-miRNA-5p was constantly negative in both saliva and plasma samples. Compared to the time before transplantation, a significant reduction in the expression of JCPyV-miRNA-5p was observed in saliva samples obtained after transplantation.

Conclusions: Altogether, these data suggest that additional investigations of polyomavirus miRNA-5p in saliva should be performed shortly after renal-transplantation to evaluate the potential role in early viral reactivation.



中文翻译:


肾移植后唾液中多瘤病毒microRNA-5p表达的检测


 抽象的


背景:多瘤病毒 (PyV) 的 MicroRNA (miRNA) 存在于多种生物体液中,被认为是监测其持久性的相关病毒因子。


目的:评估免疫抑制方案对口腔中 PyV-miRNA-5p 状态的影响。


材料和方法:使用实时 RT-PCR 对肾移植前和肾移植后不久获得的 23 名患者的配对唾液和血浆样本中的 JCPyV、BKPyV、MCPyV miRNA-5p 进行了研究。


结果:总体而言,移植后短时间内,患者出现白细胞和淋巴细胞数量减少以及肌酐水平降低的情况。在患者的临床管理过程中,与配对血浆样本(范围:9%-35%)相比,大量唾液样本 JCPyV 和 BKPyV miRNA-5p 呈阳性(范围:26%-91%)。在两种显示 miRNA-5p 阳性表达的多瘤病毒中,BKPyV 在唾液中呈现最高阳性率 (91%),而 MCPyV-miRNA-5p 在唾液和血浆样本中始终呈阴性。与移植前相比,移植后获得的唾液样本中观察到 JCPyV-miRNA-5p 的表达显着降低。


结论:总而言之,这些数据表明,应在肾移植后不久对唾液中的多瘤病毒 miRNA-5p 进行额外研究,以评估其在早期病毒再激活中的潜在作用。

更新日期:2021-03-12
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