ABSTRACT

Background: MicroRNAs (miRNAs) of polyomavirus (PyV) are present in several biological fluids and are suggested to be relevant viral factors for monitoring its persistence.

Aim: To evaluate the effect of an immunosuppressive regimen on the status of PyV-miRNA-5p in the oral cavity.

Materials and Methods: The JCPyV, BKPyV, MCPyV miRNA-5p were investigated in paired saliva and plasma samples obtained from 23 patients before and shortly after renal-transplantation by using real-time RT-PCR.

Results: Overall, within a short-time after transplantation, patients exhibited decreased numbers of leukocyte and lymphocyte as well as low levels of creatinine. During the clinical management of the patients, a significant amount of saliva samples were positive for JCPyV and BKPyV miRNA-5p (range: 26%-91%) compared to paired plasma samples (range: 9%-35%). Among the two polyomaviruses showing positive expression of miRNA-5p, BKPyV presented the highest positivity in saliva (91%) and MCPyV-miRNA-5p was constantly negative in both saliva and plasma samples. Compared to the time before transplantation, a significant reduction in the expression of JCPyV-miRNA-5p was observed in saliva samples obtained after transplantation.

Conclusions: Altogether, these data suggest that additional investigations of polyomavirus miRNA-5p in saliva should be performed shortly after renal-transplantation to evaluate the potential role in early viral reactivation.

 抽象的

背景：多瘤病毒 (PyV) 的 MicroRNA (miRNA) 存在于多种生物体液中，被认为是监测其持久性的相关病毒因子。

目的：评估免疫抑制方案对口腔中 PyV-miRNA-5p 状态的影响。

材料和方法：使用实时 RT-PCR 对肾移植前和肾移植后不久获得的 23 名患者的配对唾液和血浆样本中的 JCPyV、BKPyV、MCPyV miRNA-5p 进行了研究。

结果：总体而言，移植后短时间内，患者出现白细胞和淋巴细胞数量减少以及肌酐水平降低的情况。在患者的临床管理过程中，与配对血浆样本（范围：9%-35%）相比，大量唾液样本 JCPyV 和 BKPyV miRNA-5p 呈阳性（范围：26%-91%）。在两种显示 miRNA-5p 阳性表达的多瘤病毒中，BKPyV 在唾液中呈现最高阳性率 (91%)，而 MCPyV-miRNA-5p 在唾液和血浆样本中始终呈阴性。与移植前相比，移植后获得的唾液样本中观察到 JCPyV-miRNA-5p 的表达显着降低。

结论：总而言之，这些数据表明，应在肾移植后不久对唾液中的多瘤病毒 miRNA-5p 进行额外研究，以评估其在早期病毒再激活中的潜在作用。