A simple one-step biosynthesis route has been adopted for the synthesis of high crystalline phase pure anatase TiO₂ nanoparticles. The structural conformation and functional group analysis of the synthesized nanoparticles were made through X-Ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR), respectively. The optical property and the band gap were estimated by using UV-Visible diffuse reflectance spectroscopy (UV-DRS). The surface morphological properties of the anatase TiO₂ nanoparticles were confirmed using Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) and the size of the synthesized nanoparticles are 8 nm. The element analysis was evaluated by using EDS and X-ray photoelectron spectroscopy (XPS). The photocatalytic activity of the prepared sample was investigated by the decolourization of Methylene blue dye under UV and solar light irradiation. The maximum dye removal efficiency of 99.2% was observed for solar light irradiation. Besides, the prepared samples also exhibit excellent antibacterial activity against Klebsilla Pneumoniae and Streptococcus Pneumoniae. The antibacterial activity for the synthesized TiO₂ nanoparticles show maximum zone of inhibition (23.5 mm). Thus, the biogenic property of the bioprocessed TiO₂ nanoparticles is a potential material for environmental and biomedical applications.

已经采用一种简单的一步生物合成路线来合成高结晶相的纯锐钛矿型TiO ₂纳米粒子。分别通过X射线衍射（XRD）和傅里叶变换红外光谱（FT-IR）对合成的纳米颗粒进行结构构象和官能团分析。通过使用紫外可见漫反射光谱法（UV-DRS）估计光学性质和带隙。锐钛矿型TiO ₂的表面形貌使用扫描电子显微镜（SEM）和透射电子显微镜（TEM）确认纳米颗粒，并且合成的纳米颗粒的尺寸为8nm。元素分析通过使用EDS和X射线光电子能谱（XPS）进行评估。通过亚甲基蓝染料在紫外线和太阳光照射下的脱色研究了所制备样品的光催化活性。对于太阳光照射，观察到最大的染料去除效率为99.2％。此外，所制备的样品还显示出对肺炎克雷伯菌和肺炎链球菌的优异的抗菌活性。合成TiO ₂的抗菌活性纳米颗粒显示出最大的抑制区域（23.5毫米）。因此，经过生物处理的TiO ₂纳米颗粒的生物发生特性是用于环境和生物医学应用的潜在材料。