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A Novel Method for Unraveling the Black Box of Dissolved Organic Matter in Soils by FT-ICR-MS Coupled with Induction-Based Nanospray Ionization
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2021-03-12 , DOI: 10.1021/acs.estlett.1c00095 Shuchai Gan 1 , Pengran Guo 1 , Ying Wu 2 , Yanping Zhao 1
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2021-03-12 , DOI: 10.1021/acs.estlett.1c00095 Shuchai Gan 1 , Pengran Guo 1 , Ying Wu 2 , Yanping Zhao 1
Affiliation
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Dissolved organic matter (DOM) in soils plays important roles in carbon and nutrient cycling. However, our knowledge of the molecular composition of DOM is limited by selective loss during pretreatment [solid-phase extraction (SPE)] or small amounts of pore water. We proposed a novel method for characterizing DOM by coupling a Fourier transform ion cyclotron resonance mass spectrometer and a self-built induction-based nanospray ionizer. This approach reduces the ion suppression effect and avoids the need for SPE by substantially reducing the matrix effects. Moreover, highly diverse compound types were detected in Suwannee River natural organic matter. Cwt/Nwt was close to the actual C/N ratio. Only 0.5 μL of pore water (5 ng of C) was needed (vs ∼10 mL for the conventional method). This method was validated by analyzing forest and agricultural soil samples. Soil DOM was not dominated by lignin-like compounds, as determined using the conventional method. The novel method revealed more peptide/carbohydrate-like compounds, highly condensed aromatics, and more than double the number of CHNO compounds. A more complete understanding of DOM will allow us to elucidate the biogeochemical processes of nutrient and DOM–pollutant complexes in soils as well as to develop comprehensive policies for soil remediation.
中文翻译：
FT-ICR-MS结合基于感应的纳米喷雾电离化解土壤中溶解性有机物黑匣子的新方法
土壤中的溶解性有机物（DOM）在碳和养分循环中起着重要作用。但是，我们对DOM分子组成的了解受到预处理[固相萃取（SPE）]期间的选择性损失或少量孔隙水的限制。我们提出了一种通过耦合傅立叶变换离子回旋共振质谱仪和自建的基于感应的纳米喷雾电离器表征DOM的新方法。这种方法降低了离子抑制效应，并通过大大降低基质效应避免了对SPE的需求。此外，在苏万尼河天然有机物中还发现了多种多样的化合物类型。碳重量/氮重量接近实际的C / N比。只需0.5μL的孔隙水（5 ng C）（传统方法为约10 mL）。通过分析森林和农业土壤样品验证了该方法的有效性。使用常规方法测定，土壤DOM并非由类木质素化合物主导。该新方法显示出更多的肽/碳水化合物样化合物，高度缩合的芳族化合物，以及CHNO化合物数量增加一倍以上。对DOM的更全面的了解将使我们能够阐明土壤中的养分和DOM-污染物复合物的生物地球化学过程，并为土壤修复制定综合政策。
更新日期：2021-04-13
中文翻译：
FT-ICR-MS结合基于感应的纳米喷雾电离化解土壤中溶解性有机物黑匣子的新方法
土壤中的溶解性有机物（DOM）在碳和养分循环中起着重要作用。但是，我们对DOM分子组成的了解受到预处理[固相萃取（SPE）]期间的选择性损失或少量孔隙水的限制。我们提出了一种通过耦合傅立叶变换离子回旋共振质谱仪和自建的基于感应的纳米喷雾电离器表征DOM的新方法。这种方法降低了离子抑制效应，并通过大大降低基质效应避免了对SPE的需求。此外，在苏万尼河天然有机物中还发现了多种多样的化合物类型。碳重量/氮重量接近实际的C / N比。只需0.5μL的孔隙水（5 ng C）（传统方法为约10 mL）。通过分析森林和农业土壤样品验证了该方法的有效性。使用常规方法测定，土壤DOM并非由类木质素化合物主导。该新方法显示出更多的肽/碳水化合物样化合物，高度缩合的芳族化合物，以及CHNO化合物数量增加一倍以上。对DOM的更全面的了解将使我们能够阐明土壤中的养分和DOM-污染物复合物的生物地球化学过程，并为土壤修复制定综合政策。
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