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Isolation and identification of lignin degrading bacteria residing in mandeepkhol cave of Chhattisgarh: A RAPD Study
Indian Journal of Biochemistry and Biophysics ( IF 1.5 ) Pub Date : 2021-03-11
Arunima Sur, Kush Kumar Nayak, Piyush Parkhey, Reecha Sahu

Lignin is biopolymer synthesized by plants and it consists upto 30% dry mass weight of any woody tissue. It is also known as second most abundant source of carbon after cellulose but still has a major problem, i.e. very tough to degrade. Thus, in present work authors were studied about the degradation of lignin by bacteria. The paper reports isolation, screening and identification of lignin degrading bacteria from collected samples. The bacterial strains were isolated from a cave that is located in the Chhattisgarh state of India in the mountain range of Salewara which have a temperature range between 26-28°C. A total of 32 bacterial isolates were studied and their RAPD analysis was performed and it was observed that Brevundimonas diminuta, Bacillus thuringiensis and Bacillus cereus were isolated in lignin- rich media which showed evidence of high lignin degradation.

中文翻译:

恰蒂斯加尔邦曼德克霍尔洞穴中降解木质素的细菌的分离和鉴定:RAPD研究

木质素是植物合成的生物聚合物,占任何木质组织干重的30%。它也被认为是仅次于纤维素的第二大碳源,但仍然存在一个主要问题,。很难降解。因此,在目前的工作中,作者研究了木质素被细菌降解的情况。该论文报道了从收集到的样品中分离,筛选和鉴定木质素降解细菌的信息。细菌菌株是从位于印度萨勒瓦拉山脉Chhattisgarh州一个山洞中分离出来的,该山洞的温度范围在26-28°C之间。总共32个细菌分离进行了研究,并进行其RAPD分析和可以观察到缺陷短波单胞苏云金芽孢杆菌蜡状芽孢杆菌蜡状芽孢杆菌是在富含木质素的培养基中分离的,显示了高度木质素降解的迹象。
更新日期:2021-03-11
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