Abstract

Tumors exhibit areas of decreased oxygenation due to malformed blood vessels. This low oxygen concentration decreases the effectiveness of radiation therapy, and the resulting poor perfusion can prevent drugs from reaching areas of the tumor. Tumor hypoxia is associated with poorer prognosis and disease progression, and is therefore of interest to preclinical researchers. Although there are multiple different ways to measure tumor hypoxia and related factors, there is no standard for quantifying spatial and temporal tumor hypoxia distributions in preclinical research or in the clinic. This review compares imaging methods utilized for the purpose of assessing spatio-temporal patterns of hypoxia in the preclinical setting. Imaging methods provide varying levels of spatial and temporal resolution regarding different aspects of hypoxia, and with varying advantages and disadvantages. The choice of modality requires consideration of the specific experimental model, the nature of the required characterization and the availability of complementary modalities as well as immunohistochemistry.

 抽象的

由于血管畸形，肿瘤表现出氧合减少的区域。这种低氧浓度会降低放射治疗的有效性，并且由此产生的灌注不良会阻止药物到达肿瘤区域。肿瘤缺氧与较差的预后和疾病进展相关，因此引起临床前研究人员的兴趣。尽管有多种不同的方法来测量肿瘤缺氧和相关因素，但在临床前研究或临床中尚无量化肿瘤缺氧空间和时间分布的标准。本综述比较了用于评估临床前环境中缺氧时空模式的成像方法。成像方法针对缺氧的不同方面提供不同水平的空间和时间分辨率，并且具有不同的优点和缺点。方式的选择需要考虑具体的实验模型、所需表征的性质以及补充方式以及免疫组织化学的可用性。