Abstract

The aim of this study was to evaluate the effects of different levels of silver nanoparticles (Ag-NPs) coated on zeolite on performance, gastrointestinal microflora, heterophile to lymphocyte ratio and humoral immune system responses of broiler chickens. Three hundred seventy-five chicks (Cobb 500) in a completely randomised design were distributed to five diets: 1) basal diet, 2) basal diet containing 1% zeolite and 3, 4 and 5) basal diet containing 1% zeolite coated with 25, 50 and 75 ppm Ag-NPs. Performance traits were recorded and blood samples were taken to determine antibody response against Newcastle on day 14, infectious bursal disease on day 21 and serum immunoglobulins (Igs) were determined on day 42. Heterophile to lymphocyte ratio was evaluated on days 28, 35 and 42. The result showed that feed conversion ratio (FCR) was significantly decreased in Ag-NP treatments compared with control group (p < .05). Total anaerobic bacteria and the population of Escherichia coli in ileum and caecum were decreased (p < .05). The highest number of lactobacillus was observed in NS50 (p < .05). Antibody response against Gumboro was decreased as well as the amount of IgY antibody in Ag-NPs treatments. Significant differences were observed in heterophile to lymphocyte ratio on day 28 and 35 and the highest amount was observed in NS50 group (p < .05). In conclusion, although this study showed that using Ag-NP dietary coated on zeolite could improve gut microflora, but Ag-NPs could also have side effects on the immune mechanism of broiler chickens.

• Highlights

• Silver nanoparticles (Ag-NPs) coated on zeolite improved feed efficiency at different levels.

• Ag-NPs (50 ppm) coated on zeolite reduced Escherichia coli number and decreased lactobacillus number.

• Ag-NPs (50 ppm) coated on zeolite decreased antibody response against Gumboro and amount of IgY antibodies.

摘要

这项研究的目的是评估沸石上涂覆的不同含量的银纳米颗粒（Ag-NPs）对肉鸡生产性能，胃肠道菌群，嗜异性淋巴细胞比和体液免疫系统反应的影响。将完全随机设计的375只雏鸡（Cobb 500）分配给五种日粮：1）基础日粮，2）含1％沸石的基础日粮和3，4和5）含1％沸石的基础日粮，其中25％包被25 ，50和75 ppm的Ag-NP。记录性能特征并在第14天采集血样以确定针对新城疫的抗体反应，在第21天测定传染性法氏囊病，在第42天测定血清免疫球蛋白（Igs）。在第28、35和42天评估嗜异性细胞与淋巴细胞的比率。p  <.05）。回肠和盲肠中的总厌氧菌数量和大肠杆菌数量均减少（p  <.05）。在NS50中观察到了乳酸菌的最高数量（p  <.05）。在Ag-NPs处理中，针对Gumboro的抗体反应以及IgY抗体的量均减少。在第28天和第35天观察到嗜异性细胞与淋巴细胞的比例有显着差异，而在NS50组中观察到了最高的差异（p  <.05）。总之，尽管这项研究表明，在沸石上涂覆Ag-NP日粮可以改善肠道菌群，但是Ag-NPs也可能对肉鸡的免疫机制产生副作用。

• 强调

• 涂覆在沸石上的银纳米颗粒（Ag-NPs）在不同水平下提高了饲料效率。

• 涂在沸石上的Ag-NPs（50 ppm）减少了大肠杆菌数，减少了乳酸杆菌数。

• 涂在沸石上的Ag-NP（50 ppm）降低了针对Gumboro的抗体反应和IgY抗体的量。