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Leucine regulates porcine muscle fiber type transformation via adiponectin signaling pathway
Animal Biotechnology ( IF 1.7 ) Pub Date : 2021-03-11 , DOI: 10.1080/10495398.2021.1892709
Lu Xiang 1, 2 , Zhiqing Huang 1, 2 , Xiaoling Chen 1, 2 , Gang Jia 1, 2 , Guangmang Liu 1, 2 , Hua Zhao 1, 2
Affiliation  

Abstract

Leucine can promote slow-twitch muscle fibers formation, and this effect may be mediated by AMPK signaling pathway. In addition, adiponectin (AdipoQ) plays an important role in regulation of muscle fiber type transformation. AdipoQ is located in the upstream of AMPK and its secretion can be regulated by leucine. Therefore, the aim of this study was to explore whether leucine affects muscle fiber type transformation through AdipoQ signaling pathway. Our data showed that 4 mM leucine significantly increased protein expression levels of slow MyHC, Myoglobin, Troponin I-SS, AdipoQ, AdipoR1, phospho-AMPK (p-AMPK) and PGC-1α and mRNA expression levels of AMPKα2, PGC-1α, AdipoQ and AdipoR1, and significantly decreased fast MyHC protein expression. In addition, 4 mM leucine significantly increased the SDH activity while significantly decreased the LDH activity. However, knockdown of AdipoR1 expression by AdipoR1-siRNA abolished leucine-induced upregulation of protein expressions of slow MyHC, AdipoR1, p-AMPK, PGC-1α and NRF1, mRNA expressions of MyHC I, MyHC IIa, AdipoR1, AMPKα2 and PGC-1α, ATP5G, TFAM and NRF1, and mtDNA level, as well as downregulation of protein expression of fast MyHC and mRNA expression of MyHC IIb. Together, our data revealed that leucine promotes muscle fiber type transformation from fast-twitch to slow-twitch through AdipoQ signaling pathway.



中文翻译:

亮氨酸通过脂联素信号通路调控猪肌纤维类型转化

摘要

亮氨酸可以促进慢肌纤维的形成,这种作用可能是由AMPK信号通路介导的。此外,脂联素(AdipoQ)在调节肌纤维类型转化中起重要作用。AdipoQ位于AMPK上游,其分泌受亮氨酸调控。因此,本研究旨在探讨亮氨酸是否通过AdipoQ信号通路影响肌纤维类型转化。我们的数据显示,4 mM 亮氨酸显着增加慢速 MyHC、肌红蛋白、肌钙蛋白 I-SS、AdipoQ、AdipoR1、磷酸化 AMPK (p-AMPK) 和 PGC-1α 的蛋白质表达水平以及 AMPKα2、PGC-1α 的 mRNA 表达水平, AdipoQ 和 AdipoR1,并显着降低快速 MyHC 蛋白的表达。此外,4 mM 亮氨酸显着增加了 SDH 活性,同时显着降低了 LDH 活性。然而,通过 AdipoR1-siRNA 敲低 AdipoR1 的表达消除了亮氨酸诱导的慢速 MyHC、AdipoR1、p-AMPK、PGC-1α 和 NRF1 蛋白表达的上调,MyHC I、MyHC IIa、AdipoR1、AMPKα2 和 PGC-1α 的 mRNA 表达、ATP5G、TFAM和NRF1,以及mtDNA水平,以及fast MyHC蛋白表达和MyHC IIb mRNA表达的下调。总之,我们的数据显示,亮氨酸通过 AdipoQ 信号通路促进肌肉纤维类型从快肌向慢肌的转变。ATP5G、TFAM 和 NRF1,以及 mtDNA 水平,以及快速 MyHC 的蛋白表达和 MyHC IIb 的 mRNA 表达的下调。总之,我们的数据显示,亮氨酸通过 AdipoQ 信号通路促进肌肉纤维类型从快肌向慢肌的转变。ATP5G、TFAM 和 NRF1,以及 mtDNA 水平,以及快速 MyHC 的蛋白表达和 MyHC IIb 的 mRNA 表达的下调。总之,我们的数据显示,亮氨酸通过 AdipoQ 信号通路促进肌肉纤维类型从快肌向慢肌的转变。

更新日期:2021-03-11
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