The pore-forming activity of SsoHel308 helicase from extreme thermophilic archaea Saccharolobus solfataricus has been demonstrated for the first time. This protein embedded in rabbit erythrocyte membranes may cause erythrocyte hemolysis. It has been shown that this enzyme forms pores in a planar artificial bilayer membrane and acts as a transformer. After embedding this enzyme into biolayer lipid membranes, the membrane conductivity is altered. Taken together, our results show that SsoHel308 helicase is able to form pores in artificial bilayer membranes and, in some cases, the current that flows across the membranes shares features typical of ion channels. The short lifetime of the pores in the membrane significantly reduces the toxicity of helicase for a living cell. The possibility of directed translocation of single-stranded DNA in the presence of ATP will enable the use of this enzyme as a molecular syringe for injecting single-stranded DNA into living cells.

极端嗜热古细菌Saccharolobus solfataricus的SsoHel308解旋酶的成孔活性已首次展示。嵌入兔红细胞膜中的该蛋白可能引起红细胞溶血。已经显示出该酶在平面的人造双层膜中形成孔并充当变换器。将这种酶嵌入生物层脂质膜后，膜的电导率会发生变化。综上所述，我们的结果表明SsoHel308解旋酶能够在人造双层膜中形成孔，并且在某些情况下，流经膜的电流具有典型的离子通道特征。膜中孔的短寿命显着降低了解旋酶对活细胞的毒性。