Presently, the world is using different types of disinfectants for various applications such as disinfecting instrument surfaces in the medical field, sanitizers in airplanes, wash-rooms, etc. These disinfectants carry various harmful chemicals that harm our ecosystem. Hence in search of more eco-friendly disinfectants, our research leads us to explore plasma activated water (PAW). PAW can be produced with the help of plasma interaction with water. This interaction changes the physicochemical properties of water and makes it reactive to kill pathogens. PAW can be used for a wide variety of applications such as food preservation, fast germination, sterilization agents, etc. In the present work, PAW has been formed using non-thermal plasma and different gas or gas mixture as plasma forming gas. Its physicochemical properties have been studied to identify pH value, oxidation reduction potential, and chemical species formed after plasma interaction with a water surface. Further, the study has been carried out to determine the disinfectant ability of PAW for two types of pathogens named Staphylococcus aureus and Pseudomonas aeruginosa. Different bactericidal methods have been adopted to confirm the results. SEM, fluorescence microscopy, and release of nucleic acids and proteins from raptured cell membrane of pathogens clearly show bactericidal efficacy of PAW. The important finding of the present work is the effective killing of pathogens by PAW in a short span of interaction time (≤ 10 s). The PAW has a strong possibility of using it as a disinfectant to kill a wide variety of pathogens and micro-organisms.

当前，世界各地在各种应用中使用不同类型的消毒剂，例如医疗领域的器械表面消毒，飞机上的消毒剂，洗手间等。这些消毒剂带有各种有害化学物质，会对我们的生态系统造成危害。因此，为了寻找更环保的消毒剂，我们的研究使我们探索了血浆活化水（PAW）。PAW可以在与水的等离子体相互作用的帮助下生产。这种相互作用改变了水的理化性质，使其具有杀死病原体的活性。PAW可以用于多种应用，例如食品保存，快速发芽，杀菌剂等。在当前工作中，PAW是使用非热等离子体和不同的气体或气体混合物作为等离子体形成气体而形成的。已经对其物理化学性质进行了研究，以鉴定pH值，氧化还原电位以及等离子体与水表面相互作用后形成的化学物种。此外，已经进行了研究以确定PAW对两种类型的病原体的消毒能力。金黄色葡萄球菌和铜绿假单胞菌。已经采用了不同的杀菌方法来确认结果。扫描电镜，荧光显微镜以及病原体细胞膜中核酸和蛋白质的释放清楚地表明了PAW的杀菌功效。当前工作的重要发现是在短时间的相互作用时间内（≤10 s），PAW有效杀死了病原体。PAW极有可能用作消毒剂，以杀死各种病原体和微生物。