Abstract—

The composition and structure of the cell wall glycopolymers of Paenarthrobacter species (P. aurescens VKM Ac-1105^T, P. histidinovorans VKM Ac-1978^T, and P. nicotinovorans VKM Ac-1988^T, previously assigned to the genus Arthrobacter) were studied. All strains under study were found to contain a neutral polysaccharide, (1 → 6)-linked β-D-galactofuranan with the 2,3-diacetamido-2,3-dideoxy-α-glucopyranose (α-GlcpNAc3NAc) residues that glycosylate most of the hydroxyl groups at C-2 of galactofuranose. Acid hydrolysates of the cell walls contained galactose, glucose, arabinose, glucosamine, and galactosamine. The polysaccharide structure was established by chemical and NMR spectroscopic methods. The galactofuranan structure has not been previously described for representatives of other Actinobacteria genera. The data obtained expand our understanding of the chemical structure of microbial cell walls and may be used in taxonomy of actinobacteria, especially to differentiate at the phenotype level and to justify the description of new genera in the family Micrococcaceae.

中文翻译：

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Paenarthrobacter放线菌的细胞壁半乳糖呋喃

摘要—

的细胞壁glycopolymers的组成和结构Paenarthrobacter物种（P. aurescens VKM AC-1105 ^Ť，P. histidinovorans VKM的Ac-1978 ^Ť，和P. nicotinovorans VKM的Ac-1988 ^Ť，以前分配给属节）进行了研究。所研究的所有菌株被发现含有一个中性多糖，（1→6） -连接的β-d-galactofuranan与2,3-二乙酰-2,3-二脱氧-α-D-吡喃葡萄糖（α-GLC p（NAc3NAc）残基糖基化半乳糖呋喃糖C-2处的大部分羟基。细胞壁的酸性水解产物包含半乳糖，葡萄糖，阿拉伯糖，氨基葡萄糖和半乳糖胺。通过化学和NMR光谱法确定多糖结构。之前尚未描述过其他放线菌属的半乳呋喃聚糖结构。所获得的数据扩展了我们对微生物细胞壁化学结构的理解，可用于放线菌的分类学，尤其是在表型水平上进行区分并证明微球菌科中新属的描述是正确的。