Abstract

The present study was designed to determine the antioxidant potential of extracellular and intracellular metabolites of 130 macro/microfungi isolated from different parts of Turkey. To decide precisely and accurately, firstly, the sample ratio in a 2,2-diphenyl-1-picrylhydrazyl (DPPH) reaction mixture, culture medium, cultivation mode and extraction method of antioxidants from biomass were optimized. After optimization studies, a screening study was performed under the conditions of; potato malt peptone medium; ten days at 25°C under static cultivation mode; sample : DPPH ratio as 0.1 : 2.9 (3.33%); and microwave application for extraction of antioxidants from biomass. In the screening stage, culture liquid extracts of the vast majority of the isolates (80.77%) exhibited higher scavenging activity value than mycelial biomass extracts. Among 130 fungal isolates, six isolates (Epicoccum nigrum, Suillus collitinus, Hypomyces chrysospermus, Omphalotus olearius, Inonotus hispidus, and Pleurotus eryngii) and one isolate (Omphalotus olearius) were selected for further investigation of their extracellular and intracellular antioxidant metabolites, respectively. The selected fungal isolates had scavenging activities ranging from 50.35 to 100.00% and had phenolics ranging from 0.80 to 4.62 gallic acid equivalent mg/mL. After time-dependent DPPH radical-scavenging activity and extraction yield studies, the selected isolates were evaluated for their antioxidant activity using six different methods. As a result, Hypomyces chrysospermus was determined as the best isolate, according to high capabilities in all assay methods, except for lipid peroxidation, which was moderate. Mycelial biomass extract of the Omphalotus olearius gave impressive results for ABTS radical-scavenging activity, β-carotene bleaching and inhibition of lipid peroxidation.

中文翻译：

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土耳其菌丝体和真菌培养液抗氧化活性的筛选

摘要

本研究旨在确定从土耳其不同地区分离出的130种大型/微真菌的细胞外和细胞内代谢产物的抗氧化潜力。为了精确准确地确定，首先，优化了2,2-二苯基-1-吡啶并肼（DPPH）反应混合物中的样品比例，培养基，培养方式和从生物质中提取抗氧化剂的方法。经过优化研究后，在以下条件下进行了筛选研究：马铃薯麦芽蛋白ept培养基；在静态培养模式下于25°C放置10天；样品：DPPH比率为0.1：2.9（3.33％）; 和微波应用从生物质中提取抗氧化剂。在筛选阶段，绝大多数分离株的培养液提取物（80.77％）显示出比菌丝体生物质提取物更高的清除活性值。附球菌葵，粘盖collitinus，金孢菌寄生，Omphalotus olearius，桦褐孔菌hispidus，和杏鲍菇）和一个分离物（Omphalotus olearius）被选择用于它们的细胞外和细胞内的抗氧化代谢物的进一步调查，分别。选定的真菌分离物的清除活性为50.35至100.00％，酚类的清除率为0.80至4.62没食子酸当量mg / mL。经过时间依赖性的DPPH自由基清除活性和提取产率研究后，使用六种不同方法评估了所选菌株的抗氧化活性。结果，金丝桃属Hypomyces chrysospermus根据所有测定方法中的高功能，除脂质过氧化（中等程度）外，根据其他测定方法的最佳分离度将其确定为最佳分离物。Omphalotus olearius的菌丝体生物质提取物对于ABTS自由基清除活性，β-胡萝卜素漂白和脂质过氧化抑制作用具有令人印象深刻的结果。