From 98 Lactobacillus strains, isolated from Algerian homemade cheeses, 14 (B1-B14) were selected based on their anti-Escherichia coli and anti-Staphylococcus aureus activities. These strains were also tested towards Listeria monocytogenes 161 and Salmonella Typhimurium LT2 and further investigated for their resistance to simulated gastrointestinal digestion, cell surface properties, ability to adhere to HT-29 cells, cholesterol lowering, antioxidant activity, and technological traits. Five isolates (B9, B13, B18, B19, and B38) were active against L. monocytogenes and Salmonella. From them, three isolates, identified as Lactobacillus brevis (B9, B13, and B38) by MALDI-TOF spectrometry and 16S rDNA sequencing, exhibited high tolerance to pancreatic juice, bile salts and acidic juices, high percentages of hydrophobicity (87, 92, and 81%, respectively), auto-aggregation (61, 68, and 72%, respectively), and adherence to HT-29 cells (79, 84, and 74%, respectively), which testify on their potential of colonization of the human intestine. On the other way, the strains B9 and B13 manifested the most relevant antioxidant activity and cholesterol-lowering ability, respectively. L. brevis strains showed low acidifying and good proteolytic activities with noticeable heat tolerance. The results gathered in this study highlighted the richness of Algerian artisanal cheeses on new lactobacilli strains with an excellent probiotic potential and demonstrated that L. brevis, largely used as nonstarter in cheese manufacture, could be exploited also as a probiotic for human use.

从从阿尔及利亚自制奶酪中分离的98株乳酸杆菌中，根据其抗大肠杆菌和抗金黄色葡萄球菌的活性，选择了 14 株 (B1-B14) 。这些菌株还针对单核细胞增生李斯特菌161 和鼠伤寒沙门氏菌 LT2进行了测试，并进一步研究了它们对模拟胃肠道消化的抵抗力、细胞表面特性、粘附于 HT-29 细胞的能力、降低胆固醇、抗氧化活性和技术特性。五个分离株（B9、B13、B18、B19 和 B38）对单核细胞增生李斯特菌和沙门氏菌有活性。从它们中分离出三种分离物，鉴定为短乳杆菌（B9、B13 和 B38）通过 MALDI-TOF 光谱法和 16S rDNA 测序，对胰液、胆汁盐和酸性液具有高耐受性，疏水性百分比高（分别为 87、92 和 81%），自动聚集（分别为 61、68 和 72%）和对 HT-29 细胞的粘附（分别为 79、84 和 74%），这证明了它们在人类肠道定植的潜力。另一方面，菌株 B9 和 B13 分别表现出最相关的抗氧化活性和降低胆固醇的能力。L. brevis菌株显示出低酸化和良好的蛋白水解活性，具有明显的耐热性。本研究中收集的结果突出了阿尔及利亚手工奶酪对具有出色益生菌潜力的新乳酸杆菌菌株的丰富性，并证明了L. brevis主要用作奶酪制造中的非发酵剂，也可以用作人类使用的益生菌。