In order to study the variation of gonad lipidomics during reproductive cycle, black rockfish was employed as the research model in the present study. Using histology, lipidomics, and qPCR, the profile of gonad lipidomics and the expression levels of related genes during different developmental stages were detected and analyzed to show the potential regulatory network of lipid metabolism. Based on Ultra High-Performance Liquid Tandem Chromatography Quadrupole Time of Flight Mass Spectrometry (UHPLC-QTOFMS), four significant differential glycerophospholipid metabolic pathways including phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), and phosphatidic acid (PA) were enriched by KEGG. Pathway-related enzyme-coding genes, including phosphatidylserine decarboxylase (pisd), phosphatidylserine synthase (ptdss1, ptdss2), and phospholipase D (pld1, pld2) were identified from the whole genome data and confirmed by cloning. The expression profiles of these genes were tested by qPCR in the tissues and gonads in developmental stages, and we found that pisd, pld, and ptdss genes were all downregulated through the developmental process in the brain of male, and the latter two genes were upregulated in the liver and testis at stage IV, which were the opposite trend observed in the female. Thus, our findings would be helpful in further understanding the substance metabolism and regulation during gonad development in ovoviviparity teleosts.

为了研究生殖周期中性腺脂质组学的变化，本研究以黑石斑鱼作为研究模型。使用组织学、脂质组学和 qPCR，检测和分析性腺脂质组学的概况和不同发育阶段相关基因的表达水平，以显示脂质代谢的潜在调控网络。基于超高效液相色谱四极杆飞行时间质谱 (UHPLC-QTOFMS)，四种显着差异的甘油磷脂代谢途径包括磷脂酰胆碱 (PC)、磷脂酰乙醇胺 (PE)、磷脂酰丝氨酸 (PS) 和磷脂酸 (PA)由 KEGG 丰富。通路相关的酶编码基因，包括磷脂酰丝氨酸脱羧酶（pisd）、磷脂酰丝氨酸合酶（ptdss1、ptdss2）和磷脂酶D（pld1、pld2）是从全基因组数据中鉴定出来的，并通过克隆进行了确认。这些基因的表达谱在发育阶段的组织和性腺中通过 qPCR 检测，我们发现pisd、pld和ptdss基因在男性大脑的发育过程中全部下调，后两个基因在IV期肝脏和睾丸中上调，而在女性中观察到的趋势相反。因此，我们的研究结果将有助于进一步了解卵胎生硬骨鱼性腺发育过程中的物质代谢和调节。