Rapid quantitative analysis of single chiral amino acid (aa) was achieved using circular dichroism (CD) with data analysis by standard calibration curve. The absolute concentrations of D- and L-aas in enantiomeric mixtures were determined by CD and achiral liquid chromatography (LC) method. It is worth noting that CD and LC were used independently, not online LC/CD in this study. The errors of the experimental results were less than 10%. The method is also applicable to the quantification of non-aa chiral molecules, such as chiral nucleoside and chiral quinine. With this study, we provide a new method for the chiral quantitative analysis of enantiomeric aas mixtures.

使用圆二色性（CD）并通过标准校准曲线进行数据分析，可以快速定量分析单个手性氨基酸（aa）。通过CD和非手性液相色谱法（LC）确定对映体混合物中D-和L-aas的绝对浓度。值得注意的是，本研究中CD和LC是独立使用的，而不是在线LC / CD。实验结果的误差小于10％。该方法也适用于非氨基酸手性分子的定量，例如手性核苷和手性奎宁。通过这项研究，我们为对映体Aas混合物的手性定量分析提供了一种新方法。