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Cytogenetic analysis of Hypomasticus copelandii and H. steindachneri: relevance of cytotaxonomic markers in the Anostomidae family (Characiformes)
Comparative Cytogenetics ( IF 1.0 ) Pub Date : 2021-03-10 , DOI: 10.3897/compcytogen.v15.i1.61957
Filipe Schitini Salgado , Marina Souza Cunha , Silvana Melo , Jorge Abdala Dergam

Recent phylogenetic hypotheses within Anostomidae, based on morphological and molecular data, resulted in the description of new genera (Megaleporinus Ramirez, Birindelli et Galetti, 2017) and the synonymization of others, such as the reallocation of Leporinus copelandii Steindachner, 1875 and Leporinus steindachneri Eigenmann, 1907 to Hypomasticus Borodin, 1929. Despite high levels of conservatism of the chromosomal macrostructure in this family, species groups have been corroborated using banding patterns and the presence of different sex chromosome systems. Due to the absence of cytogenetic studies in H. copelandii (Steindachner, 1875) and H. steindachneri (Eigenmann, 1907), the goal of this study was to characterize their karyotypes and investigate the presence/absence of sex chromosome systems using different repetitive DNA probes. Cytogenetic techniques included: Giemsa staining, Ag-NOR banding and FISH using 18S and 5S rDNA probes, as well as microsatellite probes (CA)15 and (GA)15. Both species had 2n = 54, absence of heteromorphic sex chromosomes, one chromosome pair bearing Ag-NOR, 18S and 5S rDNA regions. The (CA)15 and (GA)15 probes marked mainly the subtelomeric regions of all chromosomes and were useful as species-specific chromosomal markers. Our results underline that chromosomal macrostructure is congruent with higher systematic arrangements in Anostomidae, while microsatellite probes are informative about autapomorphic differences between species.

中文翻译:

Hypomasticus copelandii和H. steindachneri的细胞遗传学分析:拟蝇科(Characiformes)中细胞分类标记的相关性

根据形态和分子数据,最近的拟吻科内的系统发育假说导致了对新属的描述(Megaleporinus Ramirez,Birindelli等人,2017年)以及其他同义物,如Leporinus copelandii Steindachner,1875年和Leporinus steindachneri Eigen的重新分配。 ,从1907年的Hypomasticus Borodin,到1929年。尽管该家族中的染色体宏观结构保守程度很高,但使用条带模式和存在不同性染色体系统的确证了物种组。由于在H. copelandii(Steindachner,1875)和H. steindachneri(Eigenmann,1907)中没有细胞遗传学研究,该研究的目的是鉴定其染色体核型,并研究使用不同重复DNA的性染色体系统的存在/不存在。探针。细胞遗传学技术包括:使用18S和5S rDNA探针以及微卫星探针(CA)15和(GA)15进行Giemsa染色,Ag-NOR条带和FISH。两种物种均具有2n = 54,不存在异型性染色体,一对具有Ag-NOR,18S和5S rDNA区域的染色体。(CA)15和(GA)15探针主要标记所有染色体的亚端粒区域,可用作物种特异性染色体标记。我们的结果强调,染色体宏观结构与拟南芥中更高的系统排列是一致的,而微卫星探针则可提供有关物种之间的拟态差异的信息。一个带有Ag-NOR,18S和5S rDNA区域的染色体对。(CA)15和(GA)15探针主要标记所有染色体的亚端粒区域,可用作物种特异性染色体标记。我们的结果强调,染色体宏观结构与拟南芥中更高的系统排列是一致的,而微卫星探针则可提供有关物种之间的拟态差异的信息。一个带有Ag-NOR,18S和5S rDNA区域的染色体对。(CA)15和(GA)15探针主要标记所有染色体的亚端粒区域,可用作物种特异性染色体标记。我们的结果强调,染色体宏观结构与拟南芥中更高的系统排列是一致的,而微卫星探针则可提供有关物种之间的拟态差异的信息。
更新日期:2021-03-10
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