Abstract

Sudan I and metanil yellow are azo dyes, illegally used to impart bright red and yellow color to foods respectively. It is assumed that these appearances are due to adulteration. Regulations must be able to detect the substances in a cost-effective, efficient, and sensitive manner. A simple and precise ultra-high-performance liquid chromatography with photodiode array detector based analytical method is developed for detection and quantification of metanil yellow and Sudan 1 from turmeric and chili powders. The method necessitated no sample clean up from matrix components and the separation of the analytes was performed on a reversed-phase C₁₈ column with an optimized solvent system consisting of 0.6305 g of ammonium formate + 100 µL of formic acid in HPLC grade water-methanol: acetonitrile (70:30 V/V) for metanil yellow at 413 nm and 0.2% formic acid in water-acetonitrile (30:70, V/V) for curcumin at 425 nm. Depending on the metanil yellow and Sudan I dye involved, LOD and LOQ were in the range of 0.05–0.12 μg/Kg and 0.3–0.5 μg/Kg in the turmeric and red chili powders respectively. The recovery, repeatability and reproducibility varied from 80.6 to 112.60% from 0.3 to 0.83%, and less than 20% RSD. Linearity obtained for metanil yellow and Sudan I was all r² > 0.9999 and in the range of 0.5–1.0 mg/L and 0.05–5 mg/L respectively. The proposed method was applied to determine the concentration of curcumin and metanil yellow in 11 market samples, 24 rhizome powder samples and Sudan1 from 9 chili powder samples. This method has the potential to be used for illegal Sudan dyes and metanil yellow in turmeric and red chili powders due to its reliable, rapid and excellent precision.

摘要

Sudan I和metanil yellow是偶氮染料，被非法用于分别赋予食品鲜艳的红色和黄色。假定这些外观是由于掺假造成的。法规必须能够以具有成本效益，高效且敏感的方式检测到这些物质。开发了一种基于光电二极管阵列检测器的简单，精确的超高效液相色谱分析方法，用于检测和定量分析姜黄粉和辣椒粉中的间苯二酚黄和苏丹红1。该方法无需从基质组分中纯化样品，并且在反相C ₁₈上进行分析物的分离色谱柱具有优化的溶剂系统，该系统由HPLC级水-甲醇：乙腈（70:30 V / V）中的HPLC级水-甲醇：乙腈（70:30 V / V）组成，用于413 nm的间甲黄和0.2％甲酸的水-乙腈（30:70，V / V）在425 nm下测定姜黄素。取决于所涉及的间位黄和苏丹红I染料，姜黄粉和红辣椒粉的LOD和LOQ分别在0.05-0.12μg/ Kg和0.3-0.5μg/ Kg的范围内。回收率，可重复性和再现性从80.6到112.60％，从0.3到0.83％不等，RSD小于20％。间苯二酚黄和苏丹红I的线性都为r ²> 0.9999，范围分别为0.5–1.0 mg / L和0.05–5 mg / L。该方法用于测定11种市售样品，24种根茎粉末样品和9种辣椒粉样品中的Sudan1中姜黄素和间苯二酚黄的浓度。该方法具有可靠，快速和出色的精度，因此有可能用于非法的苏丹染料和姜黄和红辣椒粉中的间苯二酚黄。