Abstract

The gentamicin renal toxicity has been the focal point for much discussion. The objective of this study was to investigate the impacts of Origanum vulgare L. extract and vitamin C on gentamicin dose-dependent toxicity in rats’ kidney. The present study was conducted on 60 male Wistar rats divided into ten experimental groups: control (untreated), G1, G2, G3 (100, 200, 300 mg/kg gentamicin), M1, M2 and M3 (500 mg/kg marjoram extract) + 100, 200 and 300 gentamicin, V1, V2 and V3 (Vitamin C 500 mg/kg) + 100, 200 and 300 of gentamicin. On the last day, the serum was separated from heart blood and the kidney tissues were extracted to measure the biochemical and oxidative stress parameters and histological changes. Kidney damage was confirmed as dose-dependent gentamicin by biochemical and pathological parameters. Urea, Blood Urea Nitrogen (BUN), and creatinine showed a significant increase in the G3 group compared to the control, M1, and V1 groups (p < 0.01). Catalase (CAT), Glutathione peroxidase (GPx), and Superoxide dismutase (SOD) showed a significant reduction in renal tissue in the G3 group compared to the other groups (p < 0.001). Malondialdehyde (MDA) concentration in the kidney tissue of the G3 group also showed a significant increase compared to other groups (p < 0.001). Furthermore, TNFα and IL-1 levels were the highest in the G3 group, and serum total antioxidant capacity (TAC) concentration had the lowest amount compared to other groups. Moreover, histopathological lesions of the kidney showed significant statistical differences among the groups that received gentamicin with the control and M1 group. Marjoram extract at the dose of 500 mg/kg had a desirable effect on controlling gentamicin damage in the kidneys compared with vitamin C. In particular, controlling gentamicin-induced oxidative stress and inflammation by the consumption of marjoram extract and vitamin C plays an important role in protecting the kidneys.

摘要

庆大霉素的肾毒性一直是讨论的焦点。本研究的目的是调查牛至的影响。提取物和维生素 C 对大鼠肾脏庆大霉素剂量依赖性毒性的影响。本研究对 60 只雄性 Wistar 大鼠进行，分为 10 个实验组：对照组（未处理）、G1、G2、G3（100、200、300 mg/kg 庆大霉素）、M1、M2 和 M3（500 mg/kg 马郁兰提取物） ) + 100、200 和 300 庆大霉素、V1、V2 和 V3（维生素 C 500 mg/kg）+ 100、200 和 300 庆大霉素。最后一天，从心脏血液中分离血清并提取肾脏组织，以测量生化和氧化应激参数和组织学变化。通过生化和病理参数证实肾损伤为剂量依赖性庆大霉素。与对照组、M1 和 V1 组相比，G3 组的尿素、血尿素氮 (BUN) 和肌酐显着增加（p < 0.01)。与其他组相比，G3 组的过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GPx) 和超氧化物歧化酶 (SOD) 的肾组织显着减少 ( p  < 0.001)。G3组肾组织中丙二醛（MDA）浓度也较其他组显着升高（p < 0.001)。此外，与其他组相比，G3组的TNFα和IL-1水平最高，血清总抗氧化能力（TAC）浓度最低。此外，在接受庆大霉素的组与对照组和M1组之间，肾脏的组织病理学病变显示出显着的统计学差异。与维生素 C 相比，500 mg/kg 剂量的马郁兰提取物在控制庆大霉素对肾脏的损伤方面具有理想的效果。特别是通过食用马郁兰提取物和维生素 C 来控制庆大霉素引起的氧化应激和炎症起着重要作用在保护肾脏方面。