Aim of this work is to present the phenotype and biochemical characteristics of primary freshly isolated murine hepatocytes exposed to oleic and palmitic acids, the most abundant fatty acids (FAs) in human diet and serum. Excess of FAs in the diet is one of the factors that contributes to the non-alcoholic fatty liver disease (NAFLD). The hallmark of NAFLD is the overaccumulation of lipids in hepatic tissue and formation of lipid droplets (LDs). With time NAFLD can progress into more advanced stages leading to liver injury. Hepatocytes as the most abundant cells in the liver are strongly involved in the process of LDs formation, therefore are widely used in research on molecular mechanism of NAFLD development and progression. Primary hepatocytes are rarely used in investigations, due to multistep isolation process and loss of functionality under culture conditions. However, they offer substantial advantages, including preserved phenotype and functions. In order to investigate LDs formation and their content, the Raman and FTIR imaging were employed, as they provide spatial and vibrational information on studied cells in label-free manner.

这项工作的目的是介绍暴露于油酸和棕榈酸，人类饮食和血清中最丰富的脂肪酸（FA）的新鲜新鲜分离的鼠肝细胞的表型和生化特性。饮食中FA过量是导致非酒精性脂肪肝疾病（NAFLD）的因素之一。NAFLD的标志是肝组织中脂质的过度积累和脂质滴（LDs）的形成。随着时间的流逝，NAFLD可能会进入更高级的阶段，从而导致肝损伤。肝细胞作为肝脏中最丰富的细胞，与LDs的形成过程密切相关，因此被广泛用于研究NAFLD发生和发展的分子机制。原代肝细胞很少用于研究中，由于多步隔离过程以及在培养条件下功能丧失。但是，它们具有很多优势，包括保留的表型和功能。为了研究LD的形成及其含量，采用了拉曼和FTIR成像，因为它们以无标记的方式提供了所研究细胞的空间和振动信息。