Pollen tube elongation is characterized by a highly-polarized tip growth process dependent on an efficient vesicular transport system and largely mobilized by actin cytoskeleton. Pollen tubes are an ideal model system to study exocytosis, endocytosis, membrane recycling, and signaling network coordinating cellular processes, structural organization and vesicular trafficking activities required for tip growth. Proteomic analysis was applied to identify Nicotiana tabacum Differentially Abundant Proteins (DAPs) after in vitro pollen tube treatment with membrane trafficking inhibitors Brefeldin A, Ikarugamycin and Wortmannin. Among roughly 360 proteins separated in two-dimensional gel electrophoresis, a total of 40 spots visibly changing between treated and control samples were identified by MALDI-TOF MS and LC–ESI–MS/MS analysis. The identified proteins were classified according to biological processes, and most proteins were related to pollen tube energy metabolism, including ammino acid synthesis and lipid metabolism, structural features of pollen tube growth as well modification and actin cytoskeleton organization, stress response, and protein degradation. In-depth analysis of proteins corresponding to energy-related pathways revealed the male gametophyte to be a reliable model of energy reservoir and dynamics.

花粉管伸长的特点是高度极化的尖端生长过程依赖于有效的囊泡运输系统，并且主要由肌动蛋白细胞骨架动员。花粉管是研究胞吐作用、内吞作用、膜回收和信号网络协调细胞过程、结构组织和尖端生长所需的囊泡运输活动的理想模型系统。应用蛋白质组学分析鉴定烟草用膜运输抑制剂 Brefeldin A、Ikarugamycin 和 Wortmannin 进行体外花粉管处理后的差异丰度蛋白 (DAP)。在二维凝胶电泳中分离的大约 360 种蛋白质中，通过 MALDI-TOF MS 和 LC-ESI-MS/MS 分析鉴定了总共 40 个在处理样品和对照样品之间明显变化的点。鉴定出的蛋白质根据生物学过程进行分类，大多数蛋白质与花粉管能量代谢有关，包括氨基酸合成和脂质代谢、花粉管生长的结构特征以及修饰和肌动蛋白细胞骨架组织、应激反应和蛋白质降解。对与能量相关通路相对应的蛋白质的深入分析表明，雄配子体是能量储存和动力学的可靠模型。