A simple thin-layer chromatography (TLC)–densitometric and a TLC–image analysis method were developed for the quantitative determination of an active ingredient, genistein-7-O-[α-rhamnopyranosyl-(1 → 6)]-β-glucopyranoside (GTG) in Derris scandens, by using GTG and genistein as reference standards. Due to a large polarity difference between GTG and genistein, chromatographic analysis was achieved on a silica gel 60 F₂₅₄ TLC plate using a multiple development technique, with ethyl acetate–methanol–water (8:1.3:1, V/V) and n-hexane–ethyl acetate (6:4, V/V) as the first and the second mobile phase, respectively. The method was validated and applied for the determination of GTG in D. scandens. The analytical results obtained from the TLC–densitometric, TLC–image analysis and HPLC methods were compared and found to be not significantly different.

开发了一种简单的薄层色谱法（TLC）-光密度法和TLC-图像分析法，用于定量测定有效成分genistein-7-O- [ α-鼠李吡喃糖基-（1→6）]- β-吡喃葡萄糖苷通过使用GTG和染料木黄酮作为参考标准，在Derris scandens中获得了GTG （GTG）。由于GTG和染料木黄酮之间存在很大的极性差异，因此使用多重显影技术在硅胶60 F ₂₅₄ TLC板上进行了色谱分析，其中使用乙酸乙酯-甲醇-水（8：1.3：1，V / V）和n -己烷-乙酸乙酯（6：4，V / V）分别作为第一和第二个流动阶段。验证了该方法的正确性，可用于D D中GTG的测定。比较了从TLC密度法，TLC图像分析和HPLC方法获得的分析结果，发现并没有显着差异。