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Lethal and sublethal effects of three insecticides on first instar larvae of green lacewing, Chrysoperla carnea, Stephens
International Journal of Tropical Insect Science ( IF 1.1 ) Pub Date : 2021-03-10 , DOI: 10.1007/s42690-020-00407-1
Gholamreza Golmohammadi , Hosein-Reza Rezaei Torshizi , Reza Vafaei-Shooshtari , Leila Faravardeh , Zahra Rafaei-Karehroudi

The green lacewing, Chrysoperla carnea, Stephens (Neu.: Chrysopidae) is one of the most important predators for insect pests such as whiteflies, thrips, psyllids and aphids that ignificantly decrease their populations. In the current study, lethal (LC50) and sublethal effects (LC30) of three insecticides; clothianidin, tebufenozide and flupyradifurone on first instar larvae of C. carnea (<one-day old) and their biological characteristics were evaluated using fertility life table method. Bioassays were conducted by contact method with glass petri-dishes (10 cm diameter). All experiments were conducted at controllable conditions of 25 ± 1 C°, 60 ± 5% and a photoperiod 16:8 h (L:D). The results revealed that LC50 for clothianidin, tebufenozide and flupyradifurone were 8.55, 336.12 and 642.02, respectively. Moreover, the life table response experiment shows that the values of rm and R0 due to LC30 for clothianidin, tebufenozide and flupyradifurone were 0.13, 0.13 and 0.15 per day and 95.72, 97.00 and 136.27 offspring, respectively. At the same time, rm and R0 in the control group were 0.15 per day and 143.26 offspring. Furthermore, the highest survival rate of C. carnea was observed in tebufenozide (54 days) but after control treatment, the lowest survival rate was related to clothianidin (46 days). Results reveal that longevity and life span of both sexes in three treatments were different from the control. According to the IOBC-system, comparing the total effects indicated that clothianidin (77.83%) was classified as slightly harmful while tebufenozide (20.43%) and flupyradifurone (12.89%) were categorized as harmless classes. Hence, based on obtained results, it can be concluded that flupyradifurone than clothianidin are harmless while tebufenozide is less hazardous on C. carnea population. We also measured the esterase enzyme activities of the C. carnea as biomarkers of sublethal exposure to insecticides. The results showed that alpha and beta-esterase activity in treated insects with clothianidin and flupyradifurone was increased (compared to the control), while the enzymatic activity was reduced in insects with tebufenozide treatment.



中文翻译:

三种杀虫剂对绿色草wing(Chrysoperla carnea),斯蒂芬斯(Stephens)的第一龄幼虫的致死和亚致死作用

绿色的草wing(Chrysoperla carnea),斯蒂芬斯(Stephens,Neu。:Chrysopidae)是害虫(如粉虱,蓟马,木虱和蚜虫)的重要捕食者之一,可极大地减少其种群。在目前的研究中,三种杀虫剂的致死性(LC50)和亚致死性(LC30);尼丁,虫酰肼和flupyradifurone上的第一龄幼虫C.吉祥草(<一日龄)及其生物学特性使用生育寿命表方法进行了评估。通过与玻璃陪替氏培养皿(直径为10 cm)的接触法进行生物测定。所有实验均在25±1 C°,60±5%的可控条件下以及光周期16:8 h(L:D)下进行。结果表明,可比尼丁,替布非尼嗪和氟吡呋酮的LC50分别为8.55、336.12和642.02。此外,寿命表响应实验表明,由于LC30引起的可比丁,替布芬唑和氟吡呋酮的rmR0值分别为每天0.13、0.13和0.15,后代分别为95.72、97.00和136.27。与此同时,RMR0对照组每天0.15,后代143.26。此外,最高的存活率C.吉祥草在双苯酰肼(54天),但对照处理后,观察到,最低存活率与噻虫(46天)。结果表明,三种治疗方法的男女寿命和寿命均与对照组不同。根据IOBC系统的比较,总效果表明,可比尼丁(77.83%)被分类为轻度有害,而tebufenozide(20.43%)和flupyradifurone(12.89%)被分类为无害类别。因此,根据获得的结果,可以得出结论,氟吡呋喃酮比可尼丁无害,而替布非尼嗪对角膜梭菌的危害较小人口。我们还测量了C. carnea的酯酶活性,将其作为亚致死性杀虫剂的生物标记。结果表明,用布比尼丁和氟吡呋酮处理的昆虫中的α和β-酯酶活性增加(与对照相比),而用布洛芬唑胺处理的昆虫中的酶活性降低。

更新日期:2021-03-10
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