Bifenthrin is one of the most commonly used synthetic pyrethroid insecticides. It targets the nervous system of insects, mainly acting on sodium channels in nerve cell membranes. The high use of bifrenthrin may lead to an increase in pest insect resistance. Additionally, there are only a few studies describing its cytotoxic action. A series of bioassays were carried out, and the results showed that bifenthrin has a significant ability to induce DNA damage and the inhibition of viability in Spodoptera frugiperda (Sf9) cells. Monodansylcadaverine staining and transmission electron microscope assays were used to observe significant levels of autophagosomes and mitochondrial dysfunction in the cytoplasm. Additionally, western blot analysis showed an upregulation in LC3-II and beclin-1 protein expression and a downregulation in p62 expression, which contributed to the cytotoxic effect of bifenthrin on Sf9 cells. Overall, bifenthrin significantly impacts the viability of Sf9 cells by inducing DNA damage and autophagy. These results provide a theoretical basis for understanding bifrenthin’s mechanism of cytotoxicity.

联苯菊酯是最常用的合成拟除虫菊酯杀虫剂之一。它针对昆虫的神经系统，主要作用于神经细胞膜中的钠通道。联苯菊酯的大量使用可能导致害虫抗性增加。此外，只有少数研究描述了其细胞毒性作用。进行了一系列的生物测定，结果表明联苯菊酯具有显着的诱导草地贪夜蛾DNA 损伤和抑制活力的能力(Sf9) 细胞。Monodansylcadaverine 染色和透射电子显微镜检测用于观察细胞质中显着水平的自噬体和线粒体功能障碍。此外，蛋白质印迹分析显示 LC3-II 和 beclin-1 蛋白表达上调，p62 表达下调，这有助于联苯菊酯对 Sf9 细胞的细胞毒性作用。总体而言，联苯菊酯通过诱导 DNA 损伤和自噬显着影响 Sf9 细胞的活力。这些结果为理解联苯菊酯的细胞毒性机制提供了理论基础。