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Efficacy of Chlorine Dioxide Gas Against Hepatitis A Virus on Blueberries, Blackberries, Raspberries, and Strawberries
Food and Environmental Virology ( IF 4.1 ) Pub Date : 2021-03-10 , DOI: 10.1007/s12560-021-09465-1
Bassam A Annous 1 , David A Buckley 1, 2 , David H Kingsley 3
Affiliation  

Seeking a means of sanitizing berries, the effectiveness of steady state levels of gaseous chlorine dioxide (ClO2) against hepatitis A virus (HAV) on laboratory-contaminated berries was determined. The generated ClO2 was maintained with 1 or 2 mg/l air inside a 269-l glove box to treat 50 g batches of blueberries, raspberries, and blackberries, and 100 g batches of strawberries that were immersion coated with HAV. Normalized data for ClO2 (ppm-h/g product) is reported as a function of ClO2 concentration, treatment time, and weight of treated product. Treatments of ClO2 ranging from 1.00 to 6.27 ppm-h/g berry were evaluated. When compared to untreated HAV-contaminated berries, log reductions of HAV were > 2.1 for all berry types and conditions tested indicating the gaseous ClO2 was effective. The average log reduction with strawberries, raspberries, blueberries and blackberries treated with 1.00 ppm-h/g, the lowest ClO2 treatment tested, were 2.44, 2.49, 3.23, and 3.45, respectively. The highest treatment of 6.27 ppm-h/g was applied at two different gas concentrations of 1 mg/l and 2 mg/l. Average log reductions for blueberries and strawberries treated with 6.27 ppm-h/g were 4.34 and 4.42, and 4.03 and 3.51, applied at 1 mg/l and 2 mg/l, respectively. For blackberries and raspberries 3.20 and 3.24, and 3.23 and 3.97 log reductions were observed for 6.27 ppm-h/g treatments applied at 1 mg/l and 2 mg/l, respectively. Results indicate that HAV contamination of berries can be substantially reduced by gaseous ClO2 and offer industry a waterless means of sanitizing berries against HAV.



中文翻译:

二氧化氯气体对蓝莓、黑莓、覆盆子和草莓上的甲型肝炎病毒的疗效

为寻找一种浆果消毒方法,确定了稳态水平的二氧化氯 (ClO 2 ) 对实验室污染浆果对抗甲型肝炎病毒 (HAV)的有效性。生成的 ClO 2在 269 升手套箱内保持 1 或 2 毫克/升的空气,以处理 50 克批次的蓝莓、覆盆子和黑莓,以及 100 克批次的浸涂有 HAV 的草莓。ClO 2 的归一化数据(ppm-h/g 产品)报告为 ClO 2浓度、处理时间和处理产品重量的函数。ClO 2 的处理评估范围为 1.00 至 6.27 ppm-h/g 浆果。与未经处理的受 HAV 污染的浆果相比,对于所有浆果类型和测试条件,HAV 的对数减少 > 2.1,表明气态 ClO 2是有效的。用 1.00 ppm-h/g 处理的草莓、覆盆子、蓝莓和黑莓的平均对数减少量,最低的 ClO 2测试的处理分别为 2.44、2.49、3.23 和 3.45。在 1 毫克/升和 2 毫克/升这两种不同的气体浓度下应用了 6.27 ppm-h/g 的最高处理。用 6.27 ppm-h/g 处理的蓝莓和草莓的平均对数减少分别为 4.34 和 4.42,以及 4.03 和 3.51,分别以 1 毫克/升和 2 毫克/升施用。对于黑莓和覆盆子,分别以 1 毫克/升和 2 毫克/升的浓度应用 6.27 ppm-h/g 处理,观察到 3.20 和 3.24,以及 3.23 和 3.97 对数减少。结果表明,浆果HAV污染可以通过气态CLO大幅减少2,并提供工业消毒浆果对HAV的无水手段。

更新日期:2021-03-10
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