Abstract

The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination.

中文翻译：

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新南芥菜真菌荧光素酶的合理设计和诱变

摘要

最近描述的真菌生物发光系统在开发用于生物医学研究的高效工具方面具有巨大潜力。萤光素酶是该系统最关键的组成部分之一。Neonothopanus nambi真菌的荧光素酶属于新的尚未描述的蛋白质家族。该蛋白质的结构数据几乎不存在。为了改进基于真菌生物发光系统的分析方法，需要对N. nambi荧光素酶特性进行详细研究。在这里，我们介绍使用生物信息学和实验方法描述的关键氨基酸残基的位置及其对酶功能的影响。这些结果对于进一步确定真菌荧光素酶结构是有用的。