Abstract

MLE helicase is an evolutionarily conserved eukaryotic protein involved in a wide range of processes in the regulation of gene expression. Previously, we studied the properties of MLE on the Drosophila melanogaster model. In the present work we continue studying the functions of MLE and show that MLE interacts with the components of the SWI/SNF chromatin remodelling complex. To clarify the work of MLE, the profile of MLE binding to the regulatory elements of the SWI/SNF-dependent ftz-f1 gene was analyzed. The effect of MLE on the expression of this gene, the transcription of which occurs by the RNA polymerase II pausing mechanism, was investigated. The data obtained indicate the important role of MLE in ensuring timely activation and high level of expression of the ftz-f1 gene in vivo.

中文翻译：

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MLE解旋酶是高等真核生物中编码核受体的ftz-f1基因转录调控的新参与者。

摘要

MLE解旋酶是一种进化保守的真核蛋白，参与基因表达调控的广泛过程。以前，我们在果蝇黑腹果蝇模型上研究了MLE的特性。在目前的工作中，我们继续研究MLE的功能，并表明MLE与SWI / SNF染色质重塑复合物的成分相互作用。为了阐明MLE的工作，分析了MLE与SWI / SNF依赖性ftz-f1基因的调控元件结合的情况。研究了MLE对该基因表达的影响，该转录通过RNA聚合酶II暂停机制发生。获得的数据表明MLE在确保ftz-f1的及时激活和高水平表达中的重要作用 体内基因。