Abstract

To study the rDNA contacts with genes in three human cell lines of different origin, we used 4C approach. Our data indicate that the same set of about five hundred genes frequently shape contacts with rDNA clusters in HEK293T, K652, and hESM01 cells. Gene ontology search suggests that the genes are involved in development and morphogenesis. Approximately one hundred of these genes are highly associated with silencing by H3K27me3 mark in different normal cells, including bronchial epithelial cells, keratinocytes, myoblasts, monocytes, endothelial cells, kidney epithelial cells, and some others. We conclude that the concerted silencing of specific group of rDNA-contacting genes controlling development occurs during differentiation. We assume that the phase separation mechanisms may be involved in the rDNA-mediated silencing of a set of genes via the contacts with inactive rDNA clusters.

中文翻译：

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三种人类细胞系中rDNA簇的染色体间接触与控制形态发生的基因沉默相关。

摘要

为了研究rDNA与不同来源的三种人类细胞系中的基因的接触，我们使用了4C方法。我们的数据表明，大约500个基因的同一集合经常影响HEK293T，K652和hESM01细胞中与rDNA簇的接触。基因本体搜索表明，基因参与了发育和形态发生。在不同的正常细胞中，包括支气管上皮细胞，角质形成细胞，成肌细胞，单核细胞，内皮细胞，肾上皮细胞和其他一些细胞中，约有一百个基因与H3K27me3标记的沉默高度相关。我们得出结论，分化过程中发生了控制发育的rDNA接触基因的特定组的一致沉默。