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Construction of competitive endogenous RNA network reveals regulatory role of long non-coding RNAs in intracranial aneurysm
BMC Neuroscience ( IF 2.4 ) Pub Date : 2021-03-09 , DOI: 10.1186/s12868-021-00622-7
Yuan-Bo Pan 1 , Jianan Lu 1 , Biao Yang 2 , Cameron Lenahan 3, 4 , Jianmin Zhang 1, 5, 6 , Anwen Shao 1
Affiliation  

Rupture of intracranial aneurysm (IA) is the main cause of devastating subarachnoid hemorrhage, which urges our understanding of the pathogenesis and regulatory mechanisms of IA. However, the regulatory roles of long non-coding RNAs (lncRNAs) in IA is less known. We processed the raw SRR files of 12 superficial temporal artery (STA) samples and 6 IA samples to count files. Then the differentially expressed (DE) mRNAs, miRNAs, and lncRNAs between STAs and IAs were identified. The enrichment analyses were performed using DEmRNAs. Next, a lncRNA-miRNA-mRNA regulatory network was constructed using integrated bioinformatics analysis. In summary, 341 DElncRNAs, 234 DEmiRNAs, and 2914 DEmRNAs between the STA and IA. The lncRNA-miRNA-mRNA regulatory network of IA contains 91 nodes and 146 edges. The subnetwork of hub lncRNA PVT1 was extracted. The expression level of PVT1 was positively correlated with a majority of the mRNAs in its subnetwork. Moreover, we found that several mRNAs (CCND1, HIF1A, E2F1, CDKN1A, VEGFA, COL1A1 and COL5A2) in the PVT1 subnetwork served as essential components in the PI3K-Akt signaling pathway, and that some of the non-coding RNAs (ncRNAs) (PVT1, HOTAIR, hsa-miR-17, hsa-miR-142, hsa-miR-383 and hsa-miR-193b) interacted with these mRNAs. Our annotations noting ncRNA’s role in the pathway may uncover novel regulatory mechanisms of ncRNAs and mRNAs in IA. These findings provide significant insights into the lncRNA regulatory network in IA.

中文翻译:

竞争性内源性RNA网络的构建揭示了长链非编码RNA在颅内动脉瘤中的调控作用

颅内动脉瘤(IA)破裂是破坏性蛛网膜下腔出血的主要原因,这促使我们了解IA的发病机制和调节机制。然而,长链非编码 RNA (lncRNA) 在 IA 中的调节作用鲜为人知。我们处理了 12 个颞浅动脉 (STA) 样本和 6 个 IA 样本的原始 SRR 文件以对文件进行计数。然后鉴定了STA和IA之间差异表达(DE)的mRNA、miRNA和lncRNA。使用DEmRNAs进行富集分析。接下来,使用综合生物信息学分析构建了 lncRNA-miRNA-mRNA 调控网络。总之,STA和IA之间有341个DElncRNA、234个DEmiRNA和2914个DEmRNA。IA 的 lncRNA-miRNA-mRNA 调控网络包含 91 个节点和 146 个边。提取了 hub lncRNA PVT1 的子网络。PVT1 的表达水平与其子网络中的大多数 mRNA 呈正相关。此外,我们发现 PVT1 子网中的几种 mRNA(CCND1、HIF1A、E2F1、CDKN1A、VEGFA、COL1A1 和 COL5A2)是 PI3K-Akt 信号通路的重要组成部分,一些非编码 RNA(ncRNA) (PVT1、HOTAIR、hsa-miR-17、hsa-miR-142、hsa-miR-383 和 hsa-miR-193b)与这些 mRNA 相互作用。我们注意到 ncRNA 在该通路中的作用的注释可能会揭示 IA 中 ncRNA 和 mRNA 的新调控机制。这些发现提供了对 IA 中 lncRNA 调控网络的重要见解。PVT1 子网中的 COL1A1 和 COL5A2) 作为 PI3K-Akt 信号通路的重要组成部分,并且一些非编码 RNA (ncRNA)(PVT1、HOTAIR、hsa-miR-17、hsa-miR-142、hsa -miR-383 和 hsa-miR-193b) 与这些 mRNA 相互作用。我们注意到 ncRNA 在该通路中的作用的注释可能会揭示 IA 中 ncRNA 和 mRNA 的新调控机制。这些发现提供了对 IA 中 lncRNA 调控网络的重要见解。PVT1 子网中的 COL1A1 和 COL5A2) 作为 PI3K-Akt 信号通路的重要组成部分,并且一些非编码 RNA (ncRNA)(PVT1、HOTAIR、hsa-miR-17、hsa-miR-142、hsa -miR-383 和 hsa-miR-193b) 与这些 mRNA 相互作用。我们注意到 ncRNA 在该通路中的作用的注释可能会揭示 IA 中 ncRNA 和 mRNA 的新调控机制。这些发现提供了对 IA 中 lncRNA 调控网络的重要见解。
更新日期:2021-03-09
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