Newcastle disease is an avian infectious disease caused by avian orthoavulavirus 1, also known as Newcastle disease virus (NDV). This disease has caused significant economic losses to the poultry industry worldwide. The rapid and simple detection of NDV infection is crucial to inform the appropriate control measures. We developed a reverse-transcription recombinase polymerase amplification (RT-RPA) assay combined with a lateral flow assay (LFA) for NDV detection. The RPA assay can be completed at 37°C within 20 min, and the RPA result can be visualized by the LFA within 5 min. The NDV RT-RPA-LFA detected NDV specifically with no cross-reactivity with other pathogens. The detection limit of NDV cDNA with our RT-RPA-LFA was 3.34 × 10⁻³ ng/μL. Consequently, the RT-RPA-LFA showed good potential for the detection of NDV infection in the field, especially in resource-limited settings.

新城疫是由禽原形病毒1型引起的禽类传染病，也称为新城疫病毒（NDV）。这种疾病给全世界的家禽业造成了重大的经济损失。快速简单地检测 NDV 感染对于告知适当的控制措施至关重要。我们开发了一种逆转录重组酶聚合酶扩增 (RT-RPA) 检测与横向流动检测 (LFA) 相结合的 NDV 检测。RPA 检测可在 37°C 下 20 分钟内完成，RPA 结果可在 5 分钟内通过 LFA 可视化。NDV RT-RPA-LFA 特异性检测 NDV，与其他病原体没有交叉反应。我们的 RT-RPA-LFA 对 NDV cDNA 的检测限为 3.34 × 10 ^-3 纳克/微升。因此，RT-RPA-LFA 在现场检测 NDV 感染方面显示出良好的潜力，特别是在资源有限的环境中。