The dynamics and interactions of cellular organelles underlie many aspects of cellular functioning. Until recently, assessment of organelle dynamics has been primarily observational or required whole-cell perturbations to assess the implications of altered organelle motility and positioning. However, thanks to recently developed and optimized intervention strategies, we now have the ability to control organelles in their unperturbed state, altering organelle positioning, membrane trafficking pathways, as well as organelle interactions. This can be performed both globally and locally, giving fine control over the range, reversibility, and extent of organelle dynamics. Here, we describe how these tools are currently used for controlling organelles and give insight into the exciting future of this emerging field.

细胞器的动力学和相互作用是细胞功能许多方面的基础。直到最近，细胞器动力学的评估主要是观察性的或需要全细胞扰动来评估细胞器运动和定位改变的影响。然而，由于最近开发和优化的干预策略，我们现在有能力在不受干扰的状态下控制细胞器，改变细胞器定位、膜运输途径以及细胞器相互作用。这可以在全局和局部执行，对细胞器动力学的范围、可逆性和范围进行精细控制。在这里，我们描述了这些工具目前如何用于控制细胞器，并深入了解这个新兴领域令人兴奋的未来。