Rhizobacterial diversity is an indicator of soil health and in turn it is influenced by the host crops, edaphic factors and weather. The present investigation reports the diversity of endospore forming, gram positive rhizobacteria (Bacillus megaterium, B. thuringiensis, B. cereus and Lysinibacillus spp.) inhabiting 11 different agricultural crops in the IGP of India. Twelve Bacillus thuringensis (Bt) isolates were identified by screening 62 g positive bacterial isolates from 63 rhizosperic soil samples collected from 6 districts of IGP. The highest Bt index was recorded from Bhopal (0.25) followed by Fatehpur (0.17), Kanpur dehat (0.10), Jalaun (0.08) and Hamirpur (0.06). The bacterial isolates were characterized based on the 16SrRNA gene and phylogenetically grouped into 3 clades. The spore crystal mixture of 12 Bt isolates were subjected to insect bioassay and it revealed, F8.IIPR has highest toxicity against Spilosoma obliqua Walker (100%), Olepa ricini Fabricius (91.67%) and Helicoverpa armigera Hubner (100%) larva. Survival analysis (ST₅₀) showed that the S. obliqua is highly susceptible than O. ricini and H. armigera to F8.IIPR. Crystal staining and protein profiling showed the presence of cry 1 (135 kDa) and cry 2 (65 kDa) genes in 5 Bt isolates. PCR amplification of vip3A gene confirmed its presence in five Bt isolates. To conclude F8.IIPR and Ak2.IIPR has the potential as a promising biopesticide for controlling the three lepidopteran insects tested.

根际细菌的多样性是土壤健康的指标，而根际细菌的多样性又受寄主作物，食用因子和天气的影响。本调查报告芽孢的形成多样性，革兰氏阳性生菌（巨大芽孢杆菌，苏云金芽孢杆菌，蜡样芽孢杆菌和Lysinibacillus属）在印度的IGP居住11种不同的农作物。通过从IGP 6个地区收集的63个根茎土壤样品中筛选62 g阳性细菌分离物，鉴定了12个苏云金芽孢杆菌（Bt）菌株。最高Bt博帕尔（0.25），法塔赫布尔（0.17），坎普尔·德哈特（0.10），贾劳恩（0.08）和哈米尔布尔（0.06）依次记录该指数。根据16SrRNA基因对细菌分离株进行鉴定，并在系统发育上分为3个进化枝。对12个Bt分离株的孢子晶体混合物进行了昆虫生物测定，结果显示，F8.IIPR对斜叶螺螺（100％），油epa（Olepa ricini Fabricius）（91.67％）和棉铃虫（Helicoverpa armigera Hubner）（100％）的幼虫具有最高的毒性。存活分析（ST ₅₀）表明，该小号。obliqua比O高度易感。里奇尼和H。F8.IIPR的棉铃虫。晶体染色和蛋白质谱分析显示在5个Bt分离物中存在cry 1（135 kDa）和cry 2（65 kDa）基因。vip3A基因的PCR扩增证实了它在5个Bt分离株中的存在。总而言之，F8.IIPR和Ak2.IIPR作为控制三种被测鳞翅目昆虫的有前途的生物农药具有潜力。