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Synergistic effects of D-arginine, D-methionine and D-histidine against Porphyromonas gingivalis biofilms
Biofouling ( IF 2.6 ) Pub Date : 2021-03-07 , DOI: 10.1080/08927014.2021.1893309
Zhenyang Zhang 1, 2 , Baosheng Li 1, 2 , Qing Cai 1 , Shuwei Qiao 1, 2 , Dan Wang 1, 2 , Heling Wang 3 , Huiyan Zhang 1 , Yalan Yang 2, 4 , Weiyan Meng 1
Affiliation  

Abstract

Porphyromonas gingivalis biofilms are implicated in the pathology of peri-implantitis and periodontitis. In this study, D-arginine (R), D-methionine (M), D-histidine (H), and a mixture of these D-amino acids (D-AAs) were investigated as an effective therapeutic strategy against P. gingivalis biofilms. The bacterial growth activity and minimum inhibitory concentrations were determined for each D-AA, along with the effects of the D-AAs mixture on biofilm development, morphology, structure, extracellular polysaccharides (EPS), cytotoxicity towards commensals, and bacterial structure. The D-AA mixture delayed the proliferation of P. gingivalis, changed its membrane structure, and decreased biofilm thickness and integrity, as compared with individual D-AAs. The EPS content increased with the concentration of D-AAs. The present study shows that a 4 mM RMH, triple D-AA mixture, enhanced deleterious effects on P. gingivalis biofilms without any cytotoxicity compared with individual D-AAs, thus providing a new strategy for the treatment of peri-implantitis and periodontitis.



中文翻译:

D-精氨酸,D-蛋氨酸和D-组氨酸对牙龈卟啉单胞菌生物膜的协同作用

摘要

牙龈卟啉单胞菌生物膜与种植体周围炎和牙周炎的病理有关。在这项研究中,研究了D-精氨酸(R),D-蛋氨酸(M),D-组氨酸(H)和这些D-氨基酸的混合物(D-AAs)作为对抗牙龈卟啉单胞菌的有效治疗策略生物膜。确定每种D-AA的细菌生长活性和最低抑菌浓度,以及D-AAs混合物对生物膜发育,形态,结构,细胞外多糖(EPS),对共生菌的细胞毒性和细菌结构的影响。D-AA混合物延迟了牙龈卟啉单胞菌的增殖与单独的D-AA相比,它改变了其膜结构,并降低了生物膜的厚度和完整性。EPS含量随D-AAs浓度的增加而增加。本研究表明,与单独的D-AA相比,4 mM RMH,三重D-AA混合物对牙龈卟啉单生物膜的有害作用增强,而没有任何细胞毒性,从而为种植体周围炎和牙周炎提供了新的治疗策略。

更新日期:2021-03-27
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