Abstract

Porphyromonas gingivalis biofilms are implicated in the pathology of peri-implantitis and periodontitis. In this study, D-arginine (R), D-methionine (M), D-histidine (H), and a mixture of these D-amino acids (D-AAs) were investigated as an effective therapeutic strategy against P. gingivalis biofilms. The bacterial growth activity and minimum inhibitory concentrations were determined for each D-AA, along with the effects of the D-AAs mixture on biofilm development, morphology, structure, extracellular polysaccharides (EPS), cytotoxicity towards commensals, and bacterial structure. The D-AA mixture delayed the proliferation of P. gingivalis, changed its membrane structure, and decreased biofilm thickness and integrity, as compared with individual D-AAs. The EPS content increased with the concentration of D-AAs. The present study shows that a 4 mM RMH, triple D-AA mixture, enhanced deleterious effects on P. gingivalis biofilms without any cytotoxicity compared with individual D-AAs, thus providing a new strategy for the treatment of peri-implantitis and periodontitis.

摘要

牙龈卟啉单胞菌生物膜与种植体周围炎和牙周炎的病理有关。在这项研究中，研究了D-精氨酸（R），D-蛋氨酸（M），D-组氨酸（H）和这些D-氨基酸的混合物（D-AAs）作为对抗牙龈卟啉单胞菌的有效治疗策略。生物膜。确定每种D-AA的细菌生长活性和最低抑菌浓度，以及D-AAs混合物对生物膜发育，形态，结构，细胞外多糖（EPS），对共生菌的细胞毒性和细菌结构的影响。D-AA混合物延迟了牙龈卟啉单胞菌的增殖与单独的D-AA相比，它改变了其膜结构，并降低了生物膜的厚度和完整性。EPS含量随D-AAs浓度的增加而增加。本研究表明，与单独的D-AA相比，4 mM RMH，三重D-AA混合物对牙龈卟啉单胞菌生物膜的有害作用增强，而没有任何细胞毒性，从而为种植体周围炎和牙周炎提供了新的治疗策略。