A novel chiral open tubular capillary column was fabricated with sulfobutylether-β-cyclodextrin and glycidyl methacrylate for enantioseparation in capillary electrochromatography. First, the pretreated silica-fused capillary was treated with 3-trimethoxysilyl propyl methacrylate to attach double bond ligand onto the surface. A copolymer layer was formed on the surface of capillary using glycidyl methacrylate and ethylene dimethacrylate by in situ one-pot polymerization. Sulfobutylether-β-cyclodextrin was encapsulated inside the copolymerized layer. The morphology of the developed column was characterized by field emission scanning electron microscopy. The effect of organic percentage and pH value of the mobile phase on electroosmotic flow and resolution was also investigated. The performance of the fabricated column was validated by separation of amlodipine besilate, 2,3-diphenylpropionic acid, tropic acid, and pantoprazole enantiomers with good resolutions of 3.67, 4.82, 3.34, and 2.61, respectively. The repeatabilities of column-to-column and day-to-day through relative standard deviation were found better than 4%, exhibiting satisfactory repeatability of the developed column. The results reveal that open tubular capillary columns modified with β-cyclodextrin show a great prospect for enantioseparation of chiral drugs in capillary electrochromatography.

中文翻译：

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磺丁基醚-β-环糊精固定的开放式毛细管柱在毛细管电色谱中进行手性分离

用磺基丁基醚-β-环糊精和甲基丙烯酸缩水甘油酯制备了一种新型手性开放管​​式毛细管柱，用于毛细管电色谱中的对映体分离。首先，用甲基丙烯酸3-三甲氧基甲硅烷基丙基丙酯处理预处理过的二氧化硅熔合毛细管，以将双键配体连接到表面上。使用甲基丙烯酸缩水甘油酯和二甲基丙烯酸乙二酯通过原位一锅聚合在毛细管表面上形成共聚物层。磺丁基醚-β-环糊精包封在共聚层内。通过场发射扫描电子显微镜对显影柱的形态进行了表征。还研究了流动相的有机百分比和pH值对电渗流量和分离度的影响。分离出的苯磺酸氨氯地平，2,3-二苯丙酸，对酸和pan托拉唑对映异构体的分离度分别为3.67、4.82、3.34和2.61，具有良好的分离性能，从而验证了所制备色谱柱的性能。发现色谱柱对色谱柱的重复性和每日相对标准偏差的重复性均优于4％，显示出已开发色谱柱的令人满意的重复性。结果表明，经β-环糊精修饰的敞开式毛细管柱在毛细管电色谱中手性药物的对映体分离方面具有广阔的前景。发现色谱柱对色谱柱的重复性和每日相对标准偏差的重复性均优于4％，显示出已开发色谱柱的令人满意的重复性。结果表明，经β-环糊精修饰的敞开式毛细管柱在毛细管电色谱中手性药物的对映体分离方面具有广阔的前景。发现色谱柱对色谱柱的重复性和每日相对标准偏差的重复性均优于4％，显示出已开发色谱柱的令人满意的重复性。结果表明，经β-环糊精修饰的敞开式毛细管柱在毛细管电色谱中手性药物的对映体分离方面具有广阔的前景。