Abstract

This work describes the simultaneous visualization and laser fluorescence spectroscopy of patent fingerprints on the glass slide using 405 nm laser and phase contrast microscope (PCM). High resolution images are achieved due to phase shifts conversion as light passes through a transparent specimen such as sweat layer. The residue composition can be identified by fluorescence spectroscopy depending on the wavelength used. Here, only two peaks likely corresponding to sodium chloride at 440 and 460 nm are detected. Application of fluorescein powder was found to enhance the image contrast; however when dissolved by the sweat it can play a role via fluorescence particularly when it is conjugated to appropriate contrast agents.

Graphical abstract

中文翻译：

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结合405 nm激光和相衬显微镜在载玻片上指纹的可视化和荧光光谱

摘要

这项工作描述了使用405 nm激光和相衬显微镜（PCM）在载玻片上同时对专利指纹进行可视化和激光荧光光谱分析。由于光通过透明样本（例如汗液层）时的相移转换，因此可以获得高分辨率的图像。根据所使用的波长，可以通过荧光光谱法鉴定残留物的组成。在此，仅检测到可能对应于440和460 nm氯化钠的两个峰。发现使用荧光粉可以增强图像对比度。然而，当被汗液溶解时，它可以通过荧光发挥作用，特别是当它与适当的造影剂结合时。

图形概要