A protease-producing marine bacterium, designated CF12-14(T), was isolated from sediment of the South China Sea. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain CF12-14(T) formed a separate lineage within the genus Idiomarina (Gammaproteobacteria). The isolate showed the highest 16S rRNA gene sequence similarity with Idiomarina salinarum ISL-52(T) (94.7 %), Idiomarina seosinensis CL-SP19(T) (94.6 %) and other members of the genus Idiomarina (91.9-94.6 %). Cells were gram-negative, aerobic, flagellated, straight or slightly curved, and often formed buds and prosthecae. Strain CF12-14(T) grew at 4-42 °C (optimum 30-35 °C) and with 0.1-15 % (w/v) NaCl (optimum 2-3 %). The isolate reduced nitrate to nitrite and hydrolysed DNA, but did not produce acids from sugars. The predominant cellular fatty acids were iso-C(15 : 0) (27.4 %), iso-C(17 : 0) (16.0 %) and iso-C(17 : 1)ω9c (15.8 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major respiratory quinone was ubiquinone 8. The DNA G+C content was 50.4 mol%. The phylogenetic, phenotypic and chemotaxonomic data supported the conclusion that CF12-14(T) represents a novel species of the genus Idiomarina, for which the name Idiomarina maris sp. nov. is proposed. The type strain is CF12-14(T) ( = CCTCC AB 208166(T) = KACC 13974(T)).

中文翻译：

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Idiomarina maris sp. 11月，从沉积物中分离出的一种海洋细菌

从南海的沉积物中分离出一种产生蛋白酶的海洋细菌，命名为 CF12-14(T)。16S rRNA 基因序列的系统发育分析表明，CF12-14(T) 菌株在 Idiomarina (Gammaproteobacteria) 属内形成了一个单独的谱系。该分离株与 Idiomarina salinarum ISL-52(T) (94.7 %)、Idiomarina seosinensis CL-SP19(T) (94.6%) 和 Idiomarina 属的其他成员 (91.9-94.6%) 显示出最高的 16S rRNA 基因序列相似性。细胞呈革兰氏阴性、需氧、有鞭毛、直或微弯曲，常形成芽和假体。菌株 CF12-14(T) 在 4-42°C（最佳 30-35°C）和 0.1-15% (w/v) NaCl（最佳 2-3%）下生长。分离物将硝酸盐还原为亚硝酸盐并水解 DNA，但不会从糖中产生酸。主要的细胞脂肪酸是iso-C(15:0) (27. 4%)、iso-C(17:0)(16.0%)和iso-C(17:1)ω9c(15.8%)。主要的极性脂质是磷脂酰乙醇胺、二磷脂酰甘油和磷脂酰甘油。主要的呼吸醌是泛醌8。DNA G+C 含量为50.4 mol%。系统发育、表型和化学分类数据支持以下结论：CF12-14(T) 代表 Idiomarina 属的一个新物种，其名称 Idiomarina maris sp。十一月 被提议。类型菌株是 CF12-14(T) (= CCTCC AB 208166(T) = KACC 13974(T))。表型和化学分类数据支持以下结论：CF12-14(T) 代表 Idiomarina 属的一个新物种，其名称 Idiomarina maris sp。十一月 被提议。类型菌株是 CF12-14(T) (= CCTCC AB 208166(T) = KACC 13974(T))。表型和化学分类数据支持以下结论：CF12-14(T) 代表 Idiomarina 属的一个新物种，其名称 Idiomarina maris sp。十一月 被提议。类型菌株是 CF12-14(T) (= CCTCC AB 208166(T) = KACC 13974(T))。