Partial amphiploids created by crossing octoploid tritelytrigia(2n = 8× = 56, AABBDDEE) and Thinopyrum intermedium (2n = 6× = 42, StStJJJSJS) are important intermediates in wheat breeding because of their resistance to major wheat diseases. We examined the chromosome compositions of five wheat-Th. intermedium partial amphiploids using GISH and multicolor-FISH. The result revealed that five lines had 10-14 J-genome chromosomes from Th. intermedium and 42 common wheat chromosomes, using the J-genomic DNA from Th. bessarabicum as GISH probe and the oligo probes pAs1-1, pAs1-3, AFA-4, (GAA) 10, and pSc119.2-1 as FISH probe. Five lines resembled their parent octoploid tritelytrigia (2n = 8× = 56, AABBDDEE) but had higher protein contents. Protein contents of two lines HS2-2 and HS2-5 were up to more than 20%. Evaluation of Fusarium head blight (FHB) resistance revealed that the percent of symptomatic spikelets (PSS) of these lines were below 30%. Lines HS2-2, HS2-4, HS2-5, and HS2-16 were less than 20% of PPS. Line HS2-5 with 14 J-genome chromosomes from Th. intermedium showed the best disease resistance, with PSS values of 10.8% and 16.6% in 2016 and 2017, respectively. New wheat-Th. intermedium amphiploids with the J-genome chromosomes were identified and can be considered as a valuable source of FHB resistance in wheat breeding.

中文翻译：

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5种小麦-中间产麦草部分二倍体的分子细胞遗传学特征和镰刀菌枯萎病抗性

八倍体三叶虫（2n = 8×= 56，AABBDDEE）和中间薄线虫（2n = 6×= 42，StStJJJSJS）杂交产生的部分二倍体是小麦育种的重要中间体，因为它们对主要小麦疾病具有抵抗力。我们检查了五个小麦-Th的染色体组成。使用GISH和multicolor-FISH进行中等偏二倍体。结果显示，有5个品系具有来自Th的10-14个J基因组染色体。中间和42条普通小麦染色体，使用Th的J基因组DNA。将bessarabicum作为GISH探针，将寡核苷酸探针pAs1-1，pAs1-3，AFA-4，（GAA）10和pSc119.2-1作为FISH探针。5个品系类似于它们的亲本八倍体三叶形肌痛（2n = 8×= 56，AABBDDEE），但蛋白质含量更高。两个品系HS2-2和HS2-5的蛋白质​​含量高达20％以上。对镰刀菌枯萎病（FHB）抵抗力的评估显示，这些品系的有症状小穗（PSS）的百分比低于30％。品系HS2-2，HS2-4，HS2-5和HS2-16不到PPS的20％。带有Th的14个J基因组染色体的HS2-5品系。中间媒介表现出最佳的抗病性，2016年和2017年的PSS值分别为10.8％和16.6％。新麦Th。已鉴定出具有J基因组染色体的中间间二倍体，并且可以将其视为小麦育种中FHB抗性的重要来源。新麦Th。已鉴定出具有J基因组染色体的中间间二倍体，并且可以将其视为小麦育种中FHB抗性的重要来源。新麦Th。已鉴定出具有J基因组染色体的中间间二倍体，并且可以将其视为小麦育种中FHB抗性的重要来源。