BACKGROUND:The main objective of tissue engineering is to fabricate a tissue construct that mimics native tissue both biologically and mechanically. A recurring problem for tissue-engineered blood vessels (TEBV) is deficient elastogenesis from seeded smooth muscle cells. Elastin is an integral mechanical component in blood vessels, allowing elastic deformation and retraction in response to the shear and pulsatile forces of the cardiac system. OBJECTIVE:The goal of this research is to assess the effect of the vitamin A derivative all-trans retinoic acid (RA) and polyphenol pentagalloyl glucose (PGG) on the expression of elastin in human aortic smooth muscle cells (hASMC). METHODS:A polycaprolactone (PCL) and the gelatin polymer composite was electrospun and doped with RA and PGG. The scaffolds were subsequently seeded with hASMCs and incubated for five weeks. The resulting tissue-engineered constructs were evaluated using qPCR and Fastin assay for their elastin expression and deposition. RESULTS:All treatments showed an increased elastin expression compared to the control, with PGG treatments showing a significant increase in gene expression and elastin deposition.

中文翻译：

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全反式视黄酸和五没食子酰葡萄糖对平滑肌细胞弹性生成的影响

背景：组织工程的主要目标是制造一种在生物学和机械上模拟天然组织的组织结构。组织工程血管 (TEBV) 的一个反复出现的问题是接种的平滑肌细胞缺乏弹性。弹性蛋白是血管中不可或缺的机械组件，可响应心脏系统的剪切力和脉动力而产生弹性变形和收缩。目的：本研究的目的是评估维生素 A 衍生物全反式维甲酸 (RA) 和多酚五戊酰葡萄糖 (PGG) 对人主动脉平滑肌细胞 (hASMC) 弹性蛋白表达的影响。方法：将聚己内酯 (PCL) 和明胶聚合物复合材料电纺并掺杂 RA 和 PGG。随后用 hASMC 接种支架并孵育五周。使用 qPCR 和 Fastin 测定法评估所得组织工程构建体的弹性蛋白表达和沉积。结果：与对照相比，所有处理均显示弹性蛋白表达增加，PGG 处理显示基因表达和弹性蛋白沉积显着增加。