Development of an artificial tissue by tissue engineering is witnessed to be one of the long lasting clarified solutions for the damaged tissue function restoration. To accomplish this, a scaffold is designed as a cell carrier in which the extracellular matrix (ECM) performs a prominent task of controlling the inoculated cell’s destiny. ECM composition, topography and mechanical properties lead to different types of interactions between cells and ECM components that trigger an assortment of cellular reactions via diverse sensing mechanisms and downstream signaling pathways. The polysaccharides in the form of proteoglycans and glycoproteins yield better outcomes when included in the designed matrices. Glycosaminoglycan (GAG) chains present on proteoglycans show a wide range of operations such as sequestering of critical effector morphogens which encourage proficient nutrient contribution toward the growing stem cells for their development and endurance. In this review we discuss how the glycosylation aspects are of considerable importance in everyday housekeeping functions of a cell especially when placed in a controlled environment under ideal growth conditions. Hydrogels made from these GAG chains have been used extensively as a resorbable material that mimics the natural ECM functions for an efficient control over cell attachment, permeability, viability, proliferation, and differentiation processes. Also the incorporation of non-mammalian polysaccharides can elicit specific receptor responses which authorize the creation of numerous vigorous frameworks while prolonging the low cost and immunogenicity of the substance.

通过组织工程开发人造组织被证明是修复受损组织功能的持久解决方案之一。为此，将支架设计为细胞载体，其中细胞外基质（ECM）承担着控制接种细胞命运的主要任务。ECM的成分，形貌和机械性能导致细胞与ECM组件之间发生不同类型的相互作用，从而通过多种传感机制和下游信号传导途径触发各种细胞反应。当包含在设计的基质中时，蛋白聚糖和糖蛋白形式的多糖可产生更好的效果。蛋白聚糖上存在的糖胺聚糖（GAG）链显示出广泛的操作范围，例如螯合关键效应物形态发生子，这促进了营养对生长中的干细胞的发育和耐力的充分贡献。在这篇综述中，我们讨论了糖基化方面如何在细胞的日常管家功能中具有相当重要的意义，尤其是当置于理想生长条件下的受控环境中时。由这些GAG链制成的水凝胶已被广泛用作模仿自然ECM功能的可吸收材料，以有效控制细胞附着，通透性，生存力，增殖和分化过程。