Tropospheric ozone (O₃) is a global air pollutant that causes deleterious effect to the plants. The present objective was to investigate the growth response, foliar injury, reactive oxygen species (ROS) accumulation and metabolites production in Costus pictus D. Don (insulin plant) at two developmental stages under ambient O₃ (AO) and ambient + 20 ppb O₃ (EO) using the open-top chambers (OTCs). A significant reduction in leaf area and total biomass was observed under EO as compared with AO. EO induced ROS (.O₂^– and H₂O₂) and lipid peroxidation led to more significant foliar injury and solute leakage. Image obtained from the fluorescence microscope and biochemical estimations reflected high levels of ROS under EO. A differential response in flavonoids and anthocyanin content, ascorbic acid, and antioxidative enzymes such as catalase (CAT), superoxide dismutase (SOD) and peroxidase (POX) has been observed with the growth stages of C. pictus plant. EO exposure negatively affected thiols and protein contents at all the growth stages. Secondary metabolites (tannins, lignin, saponins and alkaloids) were increased in both leaves and rhizomes due to EO, whereas phytosterols were induced only in rhizomes. Apart from other metabolites, the key bioactive compound (corosolic acid) showed its synthesis to be stimulated under EO at later growth stage. The study concludes that O₃ is a potent stimulating factor for changing the levels of secondary metabolites and antioxidants in an antidiabetic C. pictus plants as it can alter its medicinal properties.

对流层臭氧（O ₃）是一种全球性空气污染物，会对植物造成有害影响。目前的目标是研究在环境O ₃（AO）和环境+ 20 ppb O下两个阶段的野马齿tus D. Don（胰岛素植物）的生长响应，叶损伤，活性氧（ROS）积累和代谢产物的产生。₃（EO）使用敞开式腔室（OTC）。与AO相比，在EO下观察到叶面积和总生物量显着减少。EO诱导的ROS（.O ₂ ^–和H ₂ O ₂）和脂质过氧化作用导致更明显的叶损伤和溶质渗漏。从荧光显微镜获得的图像和生化估计值反映出在EO下高水平的ROS。在黄皮衣藻的生长阶段，已观察到类黄酮和花青素含量，抗坏血酸以及抗氧化酶（如过氧化氢酶（CAT），超氧化物歧化酶（SOD）和过氧化物酶（POX））的差异响应。植物。EO暴露在所有生长阶段均会对硫醇和蛋白质含量产生负面影响。由于EO，次生代谢产物（单宁，木质素，皂苷和生物碱）在叶片和根茎中均增加，而植物甾醇仅在根茎中被诱导。除其他代谢产物外，关键的生物活性化合物（椰油酸）显示其合成在生长后期处于EO刺激下。该研究得出结论，O ₃是改变抗糖尿病衣原体植物次生代谢产物和抗氧化剂水平的有效刺激因子，因为它可以改变其药用特性。