An increasing number of Bacillus strains have been developed for use as animal feed additives. The aim of the current work was to evaluate the impacts of Bacillus pumilus fsznc-09 in growth performance, organs development, blood constituents, genes expression of growth and immune in spleen and microbial communities in jejunum of weanling mice. The results showed that the body weight of mice in BP1 group increased significantly (p < 0.05) after feeding Bacillus pumilus fsznc-09. Compared with control group, the feed conversion ratio of BP1 and BP2 groups showed 13.57% (p < 0.05) and 9.64% improvements, respectively. The lengths of large intestine, small intestine in BP1 group were significantly increased (p < 0.05). While compared with control group, the organ indexes in BP1 and BP2 group did not differ significantly. Compared with control group, the activities of serum total superoxide dismutase (T-SOD), alkaline phosphatase (AKP), lysozyme (LZM) in BP1 group and T-SOD, AKP in BP2 group were significantly increased (p < 0.05). Compared with control group, the expressions of ghrelin-2 (Ghrl-2) and insulin-like growth factor 1 (Igf1) in BP1 group were significantly increased (p < 0.05). Compared with control group, the expressions of interleukin-6 (IL-6), nitric oxide synthase (INOS), tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β) in BP1 group and the expressions of IL-6, INOS, TNF-α, IL-1β and interferon alpha 11 (Ifna11) in BP2 group were slightly decreased. Moreover, compared with control group, the diversity of intestinal flora and relative abundance of potentially probiotics (e.g., Bifidobacterium, Bacillus) in BP1 and BP2 groups were increased. While compared with control group, the relative abundance of the potentially pathogenic bacterium (e.g., Staphylococcus) was reduced. The relative abundances of dominant species in BP1 (Lactobacillus johnsonii) and BP2 (Lactobacillus reuteri) groups were also higher than control group (Lactobacillus intestinalis). In conclusion, Bacillus pumilus fsznc-09 might improve the growth performance and immunity of mice.

中文翻译：

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短小芽孢杆菌对小鼠生长性能、免疫指标及肠道菌群的影响

越来越多的芽孢杆菌菌株被开发用作动物饲料添加剂。当前工作的目的是评估短小芽孢杆菌fsznc-09 对生长性能、器官发育、血液成分、脾脏生长和免疫基因表达以及断奶小鼠空肠微生物群落的影响。结果显示BP1组小鼠在 饲喂短小芽孢杆菌fsznc-09后体重显着增加（p < 0.05）。与对照组相比，BP1组和BP2组的饲料转化率 分别提高了13.57%（p <0.05）和9.64%。BP1组大肠、小肠长度显着增加（p  < 0.05)。而与对照组相比，BP1组和BP2组的器官指标没有显着差异。与对照组比较，BP1组血清总超氧化物歧化酶（T-SOD）、碱性磷酸酶（AKP）、溶菌酶（LZM）活性和BP2组T-SOD、AKP活性均显着升高（p  < 0.05）。与对照组相比，BP1组ghrelin-2（Ghrl -2）和胰岛素样生长因子1（Igf 1）的表达均显着升高（p  < 0.05）。与对照组相比，IL-6（IL -6 ）、一氧化氮合酶（INOS）、肿瘤坏死因子-α（TNF -α ）、IL-1β的表达（IL -1β）在BP1组和的表达IL -6，INOS，TNF -α，IL -1β和干扰素α11（IFNA 11）BP2组均略有下降。此外，与对照组相比，BP1和BP2组肠道菌群的多样性和潜在益生菌（如双歧杆菌、芽孢杆菌）的相对丰度有所增加。而与对照组相比，潜在致病菌（如葡萄球菌）的相对丰度有所降低。BP1（约氏乳杆菌）和BP2（罗伊氏乳杆菌）优势种的相对丰度）组也高于对照组（肠乳杆菌）。总之，短小芽孢杆菌fsznc-09 可能提高小鼠的生长性能和免疫力。