Plasma agriculture is an emerging field. In this report, we studied the effect of medium pressure (~ 10 torr) low-frequency (3–5 kHz) glow discharge (LFGD) plasma on various agronomical and physiological attributes in wheat. Wheat seeds treated by LFGD plasma generated from Argon–Oxygen (Ar + O₂) gas mixture and air showed a remarkable enhancement in seed germination by 12.38% and 7.14% respectively. Also, the morpho-physiological features significantly improved due to plasma treatment relative to control. In germinated wheat seedlings, H₂O₂ concentration markedly increased due to plasma treatments, suggesting that LFGD may be involved in nitrogen signaling in wheat. The SOD activity significantly increased in wheat seedlings subjected to Ar + O₂ plasma, while APX activity showed a significant increase only in the shoot of wheat plants in the same condition. Besides, both plasma treatments (Ar + O₂ and Air) caused a significant increase in CAT activity in the shoot but not in the root compared to non-treated plants. Analysis of wheat plants germinated from plasma treated seeds showed a notable increase in total soluble sugar and protein subjected to Ar + O₂ plasma treatment. Results also revealed a significant increase in TGM, iron, manganese, fat, and ash content while moisture content significantly decreased in wheat grain due to plasma treatments relative to controls. These findings explore the potentialities of LFGD plasma in boosting germination, growth, and nutritional traits of wheat plants.

等离子农业是一个新兴领域。在本报告中，我们研究了中压（〜10托）低频（3-5 kHz）辉光放电（LFGD）等离子体对小麦各种农艺和生理特性的影响。氩-氧气（Ar + O ₂）混合气体和空气产生的LFGD等离子体处理过的小麦种子，其种子发芽率分别显着提高了12.38％和7.14％。而且，由于血浆处理，相对于对照，形态生理特征得到显着改善。在发芽的小麦幼苗中，由于等离子体处理，H ₂ O ₂浓度显着增加，这表明LFGD可能参与了小麦的氮信号传导。Ar + O ₂胁迫下小麦幼苗的SOD活性显着增加。血浆中，而APX活性仅在相同条件下的小麦植株的芽中显示出显着增加。此外，与未处理的植物相比，两种等离子体处理（Ar + O ₂和空气）均导致苗中的CAT活性显着增加，但在根部却没有。对经过等离子体处理的种子发芽的小麦植物的分析表明，经过Ar + O ₂等离子体处理的总可溶性糖和蛋白质显着增加。结果还表明，与对照相比，由于血浆处理，小麦籽粒中TGM，铁，锰，脂肪和灰分的含量显着增加，而水分含量则显着下降。这些发现探索了LFGD血浆在促进小麦植物发芽，生长和营养性状方面的潜力。