Purpose

Cytostatic drugs cannot be easily removed by conventional sewage treatment plants, resulting in their ultimate release into aquatic systems where they become a threat. Thus, new technologies which can be used to eliminate these drugs more effectively before they enter the environment are increasingly important. Fungal treatment of wastewaters is a promising and environmentally friendly technology for pharmaceutical remediation. The aim of this work is to examine the biosorption of two cytostatics, bleomycin and vincristine, in the aqueous solution by fungal biomass.

Methods

Five white-rot fungi were used in this study: Fomes fomentarius (CB13), Hypholoma fasciculare (CB15), Phyllotopsis nidulans (CB14), Pleurotus ostreatus (BWPH), and Trametes versicolor (CB8). Tests were conducted on different types of biomass (alive and dead – autoclaved) and in various physico-chemical conditions: varied drug concentrations (5, 10 and 15 mg/L), temperatures (from 15.4 to 29.6 °C), and pH (from 3.2 to 8.8).

Results

The results showed that among alive biomass, T. versicolor (CB8) had the greatest sorption ability for bleomycin and P. nidulans (CB14) worked best for vincristine. The tested sorption process could be described by a pseudo-second order kinetics model. Sorption equilibrium studies demonstrated that for bleomycin Redlich-Peterson, while for vincristine Langmuir model fitted best. The thermodynamic studies showed that the sorption process was endothermic chemisorption for bleomycin, and exothermic physisorption for vincristine. For both drugs the sorption ability increased with an increase of the pH value.

Conclusion

The biosorption on fungal biomass is a favorable alternative to conventional wastewater treatment processes for anticancer drug removal.

中文翻译：

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白腐真菌去除两种细胞抑制药物：博来霉素和长春新碱–吸附研究

目的

常规污水处理厂无法轻松去除细胞抑制药物，从而最终将其释放到水生系统中，构成威胁。因此，可以用来在这些药物进入环境之前更有效地消除它们的新技术变得越来越重要。废水的真菌处理是一种有前途且对环境友好的药物修复技术。这项工作的目的是检查真菌生物质在水溶液中对两种抑细胞剂博来霉素和长春新碱的生物吸附作用。

方法

本研究中使用了五种白腐真菌：Fomes fomentarius（CB13），Fysholoma fasciculare（CB15），Nitrolans Phyllotopsis nidulans（CB14），Pleurotus ostreatus（BWPH）和Trametes versicolor（CB8）。在不同类型的生物质（活的和死的–高压灭菌）和各种物理化学条件下进行了测试：各种药物浓度（5、10和15 mg / L），温度（从15.4至29.6°C）和pH（ （从3.2到8.8）。

结果

结果表明，在活的生物质中，杂色丁香（CB8）对博来霉素的吸附能力最大，而构巢曲霉（CB14）对长春新碱的吸附效果最好。可以通过伪二级动力学模型描述测试的吸附过程。吸附平衡研究表明，对于博来霉素Redlich-Peterson，而对于长春新碱Langmuir模型最为合适。热力学研究表明，博来霉素的吸附过程是吸热化学吸附，长春新碱的吸附过程是放热物理吸附。对于这两种药物，吸附能力都随着pH值的增加而增加。

结论

真菌生物质上的生物吸附是用于去除抗癌药物的常规废水处理工艺的有利替代方案。