The distribution of binding agents (i.e., soil organic carbon (SOC) and glomalin-related soil protein (GRSP)) in soil aggregates was influenced by many factors, such as plant characteristics and soil properties. However, how these factors affect binding agents and soil aggregate stability along a climatic gradient remained unclear. We selected the Robinia pseudoacacia L. forests from semi-arid to semi-humid of the Loess Plateau, China to analyze the plant biomass, soil physical-chemical properties, SOC and GRSP distribution in different sized soil aggregates. We found that from semi-arid to semi-humid forests: (1) the proportion of macro-aggregates (>0.250 mm) significantly increased (P<0.05), whereas those of micro-aggregates (0.250–0.053 mm) and fine materials (<0.053 mm) decreased and soil aggregate stability was increased; (2) the contents of SOC and GRSP in macro-aggregates and micro-aggregates significantly increased, and those in fine materials decreased; (3) the contribution of SOC to soil aggregate stability was greater than those of total GRSP and easily extractable GRSP; (4) soil properties had greater influence on binding agents than plant biomass; and (5) soil aggregate stability was enhanced by increasing the contents of SOC and GRSP in macro-aggregates and soil property was the important part during this process. Climate change from semi-arid to semi-humid forests is important factor for soil structure formation because of its positive effect on soil aggregates.

结合剂（即土壤有机碳（SOC）和gloomalin相关土壤蛋白（GRSP））在土壤团聚体中的分布受多种因素的影响，例如植物特性和土壤特性。然而，这些因素如何影响结合剂和沿气候梯度的土壤团聚体稳定性尚不清楚。我们选择了黄土高原半干旱至半湿润的刺槐林，分析了不同大小土壤团聚体中的植物生物量，土壤理化性质，SOC和GRSP分布。我们发现，从半干旱到半湿润的森林：（1）大型聚集体（> 0.250 mm）的比例显着增加（P<0.05），而微骨料（0.250-0.053 mm）和细料（<0.053 mm）的含量降低，土壤骨料稳定性提高；（2）粗骨料和细骨料中的SOC和GRSP含量显着增加，细料中的SOC和GRSP含量下降；（3）SOC对土壤团聚体稳定性的贡献大于总GRSP和易于提取的GRSP；（4）土壤特性对结合剂的影响大于植物生物量；（5）通过增加大型团聚体中SOC和GRSP的含量来增强土壤团聚体的稳定性，在此过程中，土壤特性是重要的部分。从半干旱森林到半湿润森林的气候变化是土壤结构形成的重要因素，因为它对土壤团聚体具有积极作用。