Rhodopseudomonas palustris is a model microorganism for studying the anaerobic metabolism of aromatic compounds. While it is well documented which aromatics can serve as sole organic carbon sources, co-metabolism of other aromatics is poorly understood. This study used kinetic modeling to analyze the simultaneous degradation of aromatic compounds present in corn stover hydrolysates and model the co-metabolism of aromatics not known to support growth of R. palustris as sole organic substrates. The simulation predicted that p-coumaroyl amide and feruloyl amide were hydrolyzed to p-coumaric acid and ferulic acid, respectively, and further transformed via p-coumaroyl-CoA and feruloyl-CoA. The modeling also suggested that metabolism of p-hydroxyphenyl aromatics was slowed by substrate inhibition, whereas the transformation of guaiacyl aromatics was inhibited by their p-hydroxyphenyl counterparts. It also predicted that substrate channeling may occur during degradation of p-coumaroyl-CoA and feruloyl-CoA, resulting in no detectable accumulation of p-hydroxybenzaldehyde and vanillin, during the transformation of these CoA ligated compounds to p-hydroxybenzoic acid and vanillic acid, respectively. While the simulation correctly represented the known transformation of p-hydroxybenzoic acid via the benzoyl-CoA pathway, it also suggested co-metabolism of vanillic acid and syringic acid, which are known not to serve as photoheterotrophic growth substrate for R. palustris.

红假单胞菌是用于研究芳香族化合物厌氧代谢的模型微生物。尽管有充分的文献证明哪些芳族化合物可以作为唯一的有机碳源，但对其他芳族化合物的共代谢了解却很少。这项研究使用动力学模型分析了玉米秸秆水解产物中存在的芳族化合物的同时降解情况，并模拟了未知的芳香族化合物的共同代谢，而芳香族化合物作为唯一的有机底物能够支持palustris R. palustris的生长。该模拟预测将对-香豆酰酰胺和阿魏酸酰胺分别水解为对-香豆酸和阿魏酸，并通过p进一步转化。-香豆酰辅酶A和阿魏酰辅酶A。该模型还表明对-羟基苯基芳族化合物的代谢受底物抑制而减慢，而愈创木脂基芳族化合物的转化则受到其对-羟基苯基对应物的抑制。它还预测的降解过程中，可能会发生基材窜p -coumaroyl -CoA和阿魏酰辅酶A，导致没有可检测的积累p羟基苯甲醛和香草醛，这些辅酶A的转化过程中连接化合物对-羟基苯甲酸和香草酸，分别。虽然模拟正确地表示了p的已知变换经由苯甲酰-CoA途径羟基苯甲酸，它也建议香草酸和丁香酸，已知不作为光能异养生长底物的共代谢沼泽红假单胞菌。